A kind of production method of Cordyceps militaris
A production method and a technology for Cordyceps militaris are applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of short time and long time for the fruiting body of Cordyceps militaris, and achieve the advantages of improving production efficiency and shortening cultivation cycle. Effect
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preparation Embodiment 1
[0031] In this preparation example, the strain of Cordyceps militaris is cultivated in the form of liquid strain.
[0032] 200g potatoes were boiled in 1L of boiling water for 30min, filtered to obtain the potato extract, then add 20g glucose, 3g peptone, 1.5g MgSO in all the potato extract obtained above. 4 ·7H 2 O and 3 g of KH 2 PO 4 , and the volume was adjusted to 1 L, steam sterilized at 121° C. and 0.14 Mpa for 30 minutes, and then cooled to room temperature to obtain a potato liquid medium for use.
[0033] Take the Cordyceps militaris strain (purchased from Beijing Academy of Agriculture and Forestry, which is the mycelial tissue of Cordyceps militaris preserved at 4°C) with a volume of about 3mm 3 3 pieces of bacterial spawn blocks were inoculated into 150ml of the above-mentioned potato liquid medium obtained, and shaken and cultivated for 7 days at 120 revolutions per minute and a temperature of 25° C. to obtain the Cordyceps militaris liquid bacterial spawn.
preparation Embodiment 2
[0035] In this preparation example, culture materials were prepared.
[0036] 20g silkworm chrysalis is soaked in the water of 800ml for 30min and filtered to obtain the filtrate and cooled to room temperature. In all the filtrate obtained above, add the MgSO of 0.8g. 4 ·7H 2 O, 1g of KH 2 PO 4 , 6g of peptone and 60g of egg white, and dilute to 1L to obtain a basic nutrient solution.
[0037] The base material is obtained after mixing rice and soybean flour in a weight ratio of 3:1. The base material and the basic nutrient solution were mixed at a weight ratio of 1:1.2, steam sterilized at 121° C. and 0.14 Mpa for 60 minutes, and then cooled to room temperature to obtain a culture material for later use.
Embodiment 1
[0039] In the culture bottle containing the culture material obtained in Preparation Example 2 of 88g, inoculate the Cordyceps militaris bacterial classification in the liquid spawn form obtained in Preparation Example 1 of 3ml, and then carry out the cultivation of the dark culture stage.
[0040] Wherein, the culture in the dark culture stage is carried out in a light-proof environment, and the specific conditions include: the temperature is 23-25° C., and the relative air humidity can be 60%. After 9 days in the dark culture stage, it can be seen that the mycelium in the compost is basically all over the compost, and the aerial mycelium basically covers the surface of the compost, that is, the mycelium in the compost has completely penetrated the compost. At this time, the culture in the dark culture period was terminated, and then the culture in the light stimulation culture period was carried out.
[0041] Among them, the conditions of the light stimulation culture stage ...
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