High-yield strain for pneumocandin B0 and application for same

Abstract

The invention discloses a high-yield strain for pneumocandin B0, which is the mutant strain of Zalerion arboricola ATCC46001 strain, and collected in the China General Microbiological Culture Collection Centre with a collection number of CGMCC No. 5412. The invention further discloses an application for the same, wherein the yield of pneumocandin B0 prepared from the obtained high-yield strain for pneumocandin B0 achieves about 4500 mg / l.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular, it relates to a high-yield pneumocidine B0 strain and its application. Background technique [0002] In the past two decades, fungal infections that seriously endanger human life and health have been increasing in terms of mortality and types of infections, especially in immunosuppressed patients. The main drugs currently used to treat clinical deep fungal infections are azole antifungal drugs and amphotericin B. [0003] Although these two types of drugs have played an important role in controlling clinical deep fungal infections, the mortality rate of deep fungal infections remains high due to the following three deficiencies of these drugs: [0004] First, because the targets of these two types of drugs are fungal cell membranes, their selectivity is poor, resulting in relatively large toxic and side effects; [0005] The second is the reduced efficacy of these drugs du...

AI Technical Summary

Problems solved by technology

[0004] First, because the targets of these two types of drugs are fungal cell membranes, their selectivity is poor, resulting in relatively large toxic and side effects;

[0005] The second is the reduced efficacy of these drugs due to the widespread emergence of clinically resistant fungi;

[0006] The third is that these drugs are not very sensitive to fungi such as Aspergillus and Candida albicans, which makes it difficult to control the disease of this type of fungal infection clinically.

Because the fermentation yield of pneumocidine B0 is very low, and the fermentation components are complex, which in turn affects the yield of caspofungin