Application of CCDC158 gene in preparation of liver cancer treatment or diagnosis medicament

A 1. CCDC158, gene technology, applied in gene therapy, drug combination, microbial determination/examination, etc., can solve the problems of reducing the recurrence rate of liver cancer patients, and achieve the reduction of clone formation ability and cell migration rate, and cell growth rate. slow effect

Inactive Publication Date: 2013-09-11
SUN YAT SEN UNIV
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Problems solved by technology

It can be seen that clinical research alone is not enough to improve the survival rate and reduce the recurrence rate of liver cancer patients

Method used

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  • Application of CCDC158 gene in preparation of liver cancer treatment or diagnosis medicament
  • Application of CCDC158 gene in preparation of liver cancer treatment or diagnosis medicament
  • Application of CCDC158 gene in preparation of liver cancer treatment or diagnosis medicament

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Experimental program
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Embodiment

[0019] 1 Materials and methods

[0020] 1.1 Test materials

[0021] The probes and primers required for this experiment were synthesized by Guangzhou Yingjun Company. The reverse transcriptase used in the experiment was purchased from Promega Company of the United States, the qPCR kit was purchased from Invitrogen Company of the United States, the CCDC158 plasmid was purchased from Guangzhou Funeng Company, and the apoptosis detection kit was purchased from BD Company of the United States.

[0022] 1.2 SNP chip detection genotype and loss of heterozygosity

[0023] The genotypes of CCDC158 gene in tumor tissues and paracancerous tissues of 112 patients with liver cancer were detected by SNP chip. The loss of heterozygosity of the patient was judged according to the genotype of the adjacent and tumor tissue. The SNP site that is heterozygous in normal tissue is an informative case, and if the site is homozygous in tumor tissue at the same time, it is defined as the occurrenc...

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Abstract

The invention discloses the application of CCDC158 gene in preparation of liver cancer treatment or diagnosis medicament. The CCDC158 causes the loss of heterozygosity and reduced expression in a liver cancer tissue, and moreover has significant correlation with the prognosis of the liver cancer; and in the liver cancer cells, the cancer cell growth and the colony-forming ability can be reduced and the cancer cell apoptosis is induced by upregulating the expression of the CCDC158. The above shows that the gene not only has a significant effect on the occurrence and the development of the liver cancer, but also has a function of inhibiting the biology of cancer cells. Therefore, the CCDC158 can be used for preparing a new anti-tumor medicament and a kit for tumor diagnosis and prognosis evaluation.

Description

technical field [0001] The present invention relates to a new application of CCDC158 gene. Background technique [0002] Hepatocellular carcinoma (referred to as liver cancer) is one of the most common malignant tumors in my country. Studies have shown that the incidence of liver cancer has been on the rise in my country in the past two decades, and it has risen from the third place in the ranking of nine malignant tumor mortality moved up to second place. The clinical research of liver cancer has made great progress in the past ten years, but the five-year survival rate of liver cancer is still very low and the recurrence rate is high. It can be seen that clinical research alone is not enough to improve the survival rate of liver cancer patients and reduce the recurrence rate. Due to the rapid development of molecular biotechnology, the scientific community has a deeper understanding of the changes and functions of biological macromolecules in the process of tumor developm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68A61K48/00A61K45/00A61P35/00A61P1/16
Inventor 王辉云黄国良张美殷
Owner SUN YAT SEN UNIV
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