Method for extracting alisamycin from streptomyces

A technology of alisamycin and streptomyces, which is applied in the field of preparation of the antibiotic alisamycin (alisamycin), can solve the problems of adverse effects on human and animal health, has not been effectively solved, toxic and side effects, etc., and achieves good product quality and selectivity. Strong, fast extraction effects

Inactive Publication Date: 2014-12-03
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

Although the control of chemical pesticides has played an important role and achieved remarkable results, the extensive, large-scale and long-term use of chemical pesticides has brought adverse effects on human and animal health, especially the side effects and residues of chemical pesticides on humans and animals has not yet been effectively resolved

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  • Method for extracting alisamycin from streptomyces
  • Method for extracting alisamycin from streptomyces

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Embodiment Construction

[0018] Preparation of Streptomyces sansawa strain D35 fermentation broth:

[0019] Pick the spores of Streptomyces sansawa strain D35 freshly cultivated on Gao’s No. 1 slant medium and inoculate them into the seed culture medium. After pre-cultivating for 30 hours at 28°C with a rotation speed of 200r / min, transfer at an inoculum size of 8%. Into the fermentation medium, 27 ℃, rotating speed 200r / min shaking culture 72h.

[0020] Pretreatment of fermentation broth, extraction and crude extraction of active substances:

[0021] Vacuum filter 100L of fermentation broth to remove mycelium, put ethyl acetate and fermentation broth into a pear-shaped separatory funnel at a volume ratio of 2:1, fill to one-third of the separatory funnel, and cover it Stopper and shake vigorously upside down for 2 to 3 minutes, after fully contacting the organic phase and the aqueous phase, let stand to separate layers. Unscrew the top stopper, unscrew the lower piston to release the lower layer li...

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Abstract

The invention discloses a method for extracting alisamycin from streptomyces. The method comprises the steps of: with streptomyces misawanensis D35 as a fermentation strain, preparing fermentation liquor; carrying out three-time column chromatography on a crude extract which is prepared from the fermentation liquor by extraction and concentration through a silicagel column, treating by using a thin-layer point plate to obtain active ingredients; and preparing a pure product from the active ingredients by purification of a preparation-type efficient liquid chromatographic column. The pure product is identified as alisamycin, and has strong antibacterial activity on a plurality of plant pathogens, especially phytophthora nicotiana.

Description

technical field [0001] The invention relates to a method for preparing antibiotic alisamycin, in particular to a method for separating and purifying alisamycin from Streptomyces misawanensis D35. Background technique [0002] At present, chemical pesticides are mainly used for the prevention and control of plant diseases. Although the control of chemical pesticides has played an important role and achieved remarkable results, the extensive, large-scale and long-term use of chemical pesticides has brought adverse effects on human and animal health, especially the side effects and residues of chemical pesticides on humans and animals Has not been effectively resolved so far. As a result, the use of microbial metabolites to prevent and treat plant diseases has developed rapidly. [0003] Actinomycetes are a kind of extremely rich microbial resources, and they are also the type of microorganisms that produce the most antibiotic active substances. 2 / 3 of the known antibiotics s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/02C07D303/46C07D301/32C12R1/465
Inventor 朱宏建周清明刘双清周鑫钰
Owner HUNAN AGRICULTURAL UNIV
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