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Fluid culture preparing method for bamboo fungus

A technology of Dictyophora edulis and strains, which is applied in the field of liquid seed production of Dictyophora edulis, can solve the problems of fruiting body cultivation reduction, mycelium degradation, etc., to improve the efficiency of seed production, increase efficiency, and ensure the quality of strains Effect

Inactive Publication Date: 2013-09-25
黄文培
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Problems solved by technology

Since the first artificial domestication in the 1980s, high-yield and high-quality strains suitable for artificial cultivation have been selected and bred after years of production practice, and have been cultivated in large quantities in Fujian, Sichuan and other provinces and cities. At present, the main method of seed production is to use bamboo residue solid medium To prepare primary and secondary cultivars, it takes 4-5 labors and 8-12 months to prepare 50 tons of cultivars, and after many times of extended cultivation and transfer, it will easily lead to the degradation of mycelium and the reduction of fruiting body cultivation

Method used

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Examples

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Effect test

Embodiment 1

[0012] Take 950 g of commercially available mildew-free wheat grains, wash them 2-3 times, soak them in clean water at room temperature for 12 hours, filter and rinse them for 3 times, add water to boil until the wheat grains have no white heart, filter them, add cold water to cool to room temperature, and filter them. Add 50 g of miscellaneous sawdust to the wheat grains and mix well, the pH is natural, and equally divided into four 500 ml Erlenmeyer flasks, sterilized at 121°C for 30 minutes, cooled to room temperature, and each bottle was inserted into a test tube to preserve the strains (mycelia The wet weight is about 0.5-1 g), shake the Erlenmeyer flask lightly, so that the bacteria are evenly distributed among the wheat grains, and cultivate at a constant temperature of 25°C until the mycelia cover the Erlenmeyer flask.

[0013] Take a 250 ml Erlenmeyer flask, sterilize the empty bottle at 121°C for 20 minutes, transfer 5 g of the above-mentioned strains of wheat grains ...

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Abstract

The invention provides a culture preparing method for bamboo fungus, which includes the following steps: adopting a kernel solid medium for culture activation under proper culture condition, adopting soybean fluid medium to cultivate activated cultures, and finally obtaining a bamboo fungus fluid culture with uniform and dense mycelium pellets with proper sizes, then inoculating the culture to a bamboo slag solid medium for cultivation, and obtaining the cultivated culture. According to the method, time in culture preparation is shortened, the culture preparation efficiency is improved, and the cultivated culture is suitable for traditional cultivation patterns.

Description

technical field [0001] The present invention relates to a liquid seed production method of Dictyophora long skirt, mainly inoculating Dictyophora long skirt strains into soybean liquid culture medium of a certain formula, forming a large number of small mycelial balls by using the shaker action, shortening the seed production time, Improve seed production efficiency. [0002] Background technique [0003] Dictyophora long skirt, also known as bamboo ginseng and net gauze fungus, belongs to Basidiomycota, Basidiomycetes, Phallogramles, and Phallaceae. It is a precious edible and medicinal fungus. Since the first artificial domestication in the 1980s, high-yield and high-quality strains suitable for artificial cultivation have been selected and bred after years of production practice, and have been cultivated in large quantities in Fujian, Sichuan and other provinces and cities. At present, the main method of seed production is to use bamboo residue solid medium To prepare p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A01G31/00
Inventor 黄文培游玲王超
Owner 黄文培
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