Preparation method for beef essence
A technology of essence and beef, which is applied in the food field to achieve the effect of full flavor
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Embodiment 1
[0015] Preparation of broad bean protein hydrolyzate: Accurately weigh 6 broad bean protein isolates, dissolve in 100mL distilled water, adjust the solution temperature to 80°C, pH to 8.0, pre-treat for 15min, add 96000U alkaline protease, at a temperature of 50°C, pH value The hydrolysis reaction is carried out under the condition of 8.0, and the hydrolysis time is 1h. After reaching the time, adjust the pH value of the solution to 4.0, and maintain it in a water bath at 85°C for 20min to inactivate the enzyme, and then quickly cool to room temperature, at 4000rpm / min Centrifuge for 20 minutes, pour out the supernatant, and adjust the pH to 7.0 for later use;
[0016] Add 70% of broad bean protein hydrolyzate, 12.5% of glucose, 3% of cysteine, 2% of thiamine, 2.5% of taurine, 3% of tallow, 0.2% of NaCl and 0.7% of compound amino acid in the reaction kettle. Stir evenly, heat, the temperature is 100°C, and react for 150 minutes. After the reaction is completed, lower the tem...
Embodiment 2
[0018] Add 50% of broad bean protein hydrolyzate, 12.5% of glucose, 1% of cysteine, 1% of thiamine, 2.5% of taurine, 3% of tallow, 0.2% of NaCl and 0.7% of compound amino acid into the reaction kettle successively. The initial pH was 6.0, stirred evenly, heated at 100°C, and reacted for 120 minutes. After the reaction was completed, the temperature was lowered and cooled to obtain a meaty flavor with a realistic beef flavor. The aroma components were analyzed by SPME-GC-MS (solid phase microextraction-gas-mass spectrometry), and it was found that aldehydes (67.164%), furans (0.368%), thiophenes (0.289%), thiophenones were detected Thirty alcohols (5.083%), mercaptans (20.773%) and other ketones, alcohols and other 30 compounds that contribute to the taste of meat. Among them, furan compounds and their derivatives contribute greatly to the aroma of meat. In addition, 2-ethyl-6-methylpyrazine is a compound that contributes significantly to the characteristic meat flavor of be...
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