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Family-planning four-item combined kit employing enzyme linked immunosorbent spot assay

An enzyme-linked immunospot and kit technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inconvenient portability and operation, no spot reaction plate, complicated detection process, etc., to achieve easy popularization and novel design , the effect of simple operation

Inactive Publication Date: 2013-11-20
QINGDAO ZHONGREN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, when the immunospot method is used to detect and diagnose these four diseases, the detection of each disease is completed separately, and all kinds of reagents are scattered and placed. There is no special spot reaction plate, so the detection process is complicated, time-consuming, and difficult to carry and operate. convenient

Method used

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  • Family-planning four-item combined kit employing enzyme linked immunosorbent spot assay
  • Family-planning four-item combined kit employing enzyme linked immunosorbent spot assay

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Such as figure 1 As shown, the present invention is provided with a carrier plate 1, a nitrocellulose membrane 2 pasted on the surface of the carrier plate 1, a cytomegalovirus antibody (antigen) detection point 3, a toxoplasma antibody (antigen) detection point 4, a rubella virus antibody (antigen) detection point ) detection points 5, herpes simplex virus antibody (antigen) detection points 6, and control points 7 are sequentially arranged on the nitrocellulose membrane 2 from top to bottom, and incubation tanks 8 are formed around each detection line point and control point; Corresponding antigens ( antibody); at control point 7, there are four control points 9-12 from left to right, respectively coated with cytomegalovirus antigen, toxoplasma gondii antigen, rubella virus antigen, herpes simplex virus antigen or coated with cytomegalovirus antibody , Toxoplasma gondii antibody, rubella virus antibody, herpes simplex virus antibody positive serum.

[0021] Such as ...

Embodiment 2

[0031] Example 2, the method of using the four combined kits of enzyme-linked immunospot family planning of the present invention

[0032] 1. The sample to be tested is diluted 1:50 with 0.01mol / L PBS buffer.

[0033] 2. Take out the carrier plate, pipette 0.2mL-0.3mL of the sample to be tested, put it into the incubation tank, and incubate at room temperature (18-25)°C for 30min.

[0034] 3. Aspirate the liquid in the incubation tank, wash each incubation tank with 2mL 0.01mol / L PBS buffer solution 3 times, 5min each time.

[0035]4. Add enzyme conjugates: Add 0.2mL-0.3mL enzyme conjugates (use alkaline phosphatase-labeled secondary antibody (corresponding antibody) or horseradish peroxidase-labeled secondary antibody (corresponding antibody) into the incubation tank respectively antibody)), incubate at 18-25°C for 30min.

[0036] 5. Aspirate the liquid in the tank, wash each incubation tank with 2mL0.01mol / L PBS buffer solution 3 times, 5min each time.

[0037] 6. Substra...

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Abstract

A family-planning four-item combined kit employing enzyme linked immunosorbent spot assay is provided with a carrier board, cytomegalovirus, toxoplasma, rubella virus, a herpes simplex virus antibody / antigen detection line / point, a contrast line / point, a cellulose nitrate membrane and the like; the cellulose nitrate membrane is pasted on the surface of the carrier board; cytomegalovirus, toxoplasma, rubella virus, the herpes simplex virus antibody / antigen detection line / point and the contrast line / point are successively disposed on the cellulose nitrate membrane from top to bottom; cytomegalovirus, toxoplasma, rubella virus, and the herpes simplex virus antibody / antigen detection line / point are coated by corresponding antigens or antibodies; and the contrast line / point is coated by cytomegalovirus, toxoplasma, rubella virus and herpes simplex virus antigen, or is coated by a patient positive serum comprising cytomegalovirus, toxoplasma, rubella virus and herpes simplex virus antibody. The family-planning four-item combined kit is capable of rapidly detecting family-planning four items by one operation, is simple in operation, low in cost, novel in design and easy to observe, and helps to make a diagnosis.

Description

Technical field: [0001] The invention relates to a kit for one-time detection of four family planning viruses (species) by enzyme-linked immunospot technology, and belongs to the technical field of detection of four family planning combined kits for medical diagnosis. Background technique: [0002] The four family planning tests are an important test for women of childbearing age in early pregnancy to prevent birth defects. It is of great significance for improving the quality of the newborn population and reducing the risk of newborn births. It has been approved by medical institutions and Widely accepted and applied by pregnant women. Four family planning tests included rubella virus, cytomegalovirus, toxoplasma, and herpes simplex virus. Through the four family planning tests, it is possible to detect whether there is infection in the pregnant woman and the fetus, and to treat or terminate the pregnancy in a targeted manner. [0003] At present, when the immunospot meth...

Claims

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Application Information

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IPC IPC(8): G01N33/569
Inventor 苏冬梅张述智夏伟
Owner QINGDAO ZHONGREN PHARMA
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