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Method for making tobacco rhizome disease specimens

A technology for disease specimens and production methods, which is applied in the fields of botanical equipment and methods, plant preservation and application, can solve problems such as loss of tobacco leaves, aggravation of diseases, inability to accurately grasp the symptoms of damage, etc. Effects of damage to loss

Inactive Publication Date: 2013-11-27
湖北省烟草公司恩施州公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the technical training and teaching on the prevention and control of tobacco leaf root and stem diseases are mainly in the form of text, wall charts, photos, etc. In this way, what the students see is only a certain aspect of the pictures and knowledge content, but they cannot accurately grasp the symptoms of tobacco leaf root and stem diseases. In the process of tobacco leaf production, technicians cannot correctly diagnose the types of diseases on the roots and stems of tobacco leaves and prevent and control them, and miss the best control period and scientific use of chemicals, resulting in aggravated diseases and serious losses of tobacco leaves; technical training can be carried out by making physical specimens of diseases on the roots and stems of tobacco leaves. The disease symptoms and pathogens are explained in detail to improve the training effect, so that technicians can master the types, diagnosis and control methods of root and stem diseases, and provide technical support for tobacco leaf production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation method of a kind of tobacco leaf rhizome disease specimen of the present invention comprises the following steps:

[0036] a. Collection and pretreatment of tobacco leaf root disease specimens

[0037] Select the stems of tobacco plants with disease-type symptoms on the root and stem of tobacco leaves, keep the intact root system and cut them together with healthy tobacco stems 10cm away from the disease, remove the root soil and write a label, save the disease-type symptoms and wrap them in newspaper, and put the freshly harvested tobacco stems Tobacco root and stem disease specimens are taken back indoors for further cleaning, excess roots and stems are removed, and the dirt is washed away for later use;

[0038] b. Fixative solution kills and fixes

[0039] Put the diseased specimens of the roots and stems of the tobacco leaves cleaned in the above step into the fixative solution for killing, fixing and color preservation. The fixing time is 6-24 hou...

Embodiment 2

[0043]A method for preparing a specimen of tobacco leaf root disease in the present invention takes the preparation of a specimen of tobacco leaf bacterial wilt as an example.

[0044] a. At the early stage of the disease, the leaves are withered and still green, the leaves on the diseased side are withered, the disease-free side is normal, and the branch roots on one side are black and rotten, showing the symptoms of "half madness" and the stem epidermis in the middle and late stages of the disease. Black and rotten, the roots also turn black and rot, duct has 5 strains of tobacco plants with yellow-white bacterial pus and black symptoms, remove the root soil and write on the label, and wrap it with newspaper to facilitate the preservation of the disease-type symptoms. Bring it back indoors for further cleaning, removing excess roots and stalks, and rinsing off the dirt for later use.

[0045] b. Put the cleaned tobacco leaf root bacterial wilt specimen into the fixative solu...

Embodiment 3

[0048] A method for preparing a specimen of tobacco leaf rhizome disease in the present invention takes the preparation of a specimen of tobacco black shank as an example.

[0049] A. In the early stage of the disease, there are black spots at the base of the stem, and large round spots of "black plaster" on the middle and lower leaves, and 5 tobacco plants each with shrunken pith in the shape of a "disk". Remove the root soil and write labels, and wrap them in newspapers. Well, it is convenient to save the symptoms of the disease. Take it back indoors for further cleaning, remove excess roots and stems, and rinse off the dirt for later use.

[0050] B. Put the cleaned specimen into the fixative solution for biocidal fixation and color preservation. The fixation time for leaves is 6 hours, and the fixation time for roots and stems is 24 hours, depending on the size of the disease specimens, and the specific standard is until the color of the disease symptoms is stable. The fo...

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Abstract

The invention relates to a method for making tobacco rhizome disease specimens, which is characterized by comprising the following steps: a, collecting and pretreating tobacco rhizome disease specimens; b, killing and fixing the specimens by using stationary liquid; and c, preserving the specimens by using preserving fluid. Tobacco rhizome disease specimens made by using the method can show the damage symptoms of different parts and different times; the tobacco rhizome disease specimens can used as the best and most intuitive teaching aids and observation materials in the training and teaching of tobacco rhizome disease prevention and control technologies, thereby grasping the disease specimens of different tobacco rhizome diseases in the fields and the types of tobacco rhizome diseases more clearly.

Description

technical field [0001] The invention relates to a method for preparing a disease specimen of tobacco leaf roots and stems. Background technique [0002] Specimens are very important, even indispensable, in collection, teaching and research, so the production of specimens has always been valued by people. At present, there are many ways to prepare specimens, among which soaking specimens has the best effect. The so-called soaking refers to a type of fresh plant that is quickly killed and fixed in a fixative solution, and then transferred to a preservation solution for sealed storage. specimen. Compared with crude drug specimens and cured leaf specimens, soaked specimens have the advantages of no decay, no insects, long storage period, and realistic shapes, effectively preserving the original forms of multiple organs of the original plant. Therefore, immersed specimens have considerable advantages in practical teaching. There are few units in China that can make soaked spec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00
Inventor 谭军王瑞孟贵星向必坤施河丽吴文昊左梅霍光
Owner 湖北省烟草公司恩施州公司
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