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An anti-caries enamel matrix protein functional polypeptide and its preparation method and application

A technology of enamel matrix protein with multiple functions, which is applied in the field of anti-caries enamel matrix protein functional polypeptides and its preparation, can solve the problems of complex genetic recombinant protein procedures, imperfect processing and modification systems, and difficulty in obtaining high-purity products, etc., to achieve good results. Safety and biocompatibility, increased local action time, cost-effective effect

Active Publication Date: 2014-10-08
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the above-mentioned enamel matrix protein sources, the extraction method requires more tissue materials and obtains mixed components of different molecular weights, making it difficult to obtain high-purity products; the procedure for gene recombinant protein is complicated, and the post-translational processing and modification system is not perfect. Difficult, often imperfect protein function; Sweden The enamel matrix protein extracted from porcine tooth germ is purified and obtained by freeze-drying and storing the three relative molecular weight peaks of typical amelogenin. Although it has the effect of promoting periodontal tissue regeneration, it may lose the effective anti-caries functional fragment
To sum up, the current method of obtaining enamel matrix protein is complex and may lose effective anti-caries fragments. How to use enamel matrix protein for effective caries prevention has become a difficult problem that has been wanted to be solved in this field but has not been solved.

Method used

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  • An anti-caries enamel matrix protein functional polypeptide and its preparation method and application
  • An anti-caries enamel matrix protein functional polypeptide and its preparation method and application
  • An anti-caries enamel matrix protein functional polypeptide and its preparation method and application

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Embodiment 1

[0037]An anti-caries polypeptide, the amino acid sequence of which is shown in SEQ ID NO.1;

[0038] SEQ ID NO.1: QPYQPVQPHQPMQPQ

Embodiment 2

[0040] An anti-caries polypeptide, the amino acid sequence of which is shown in SEQ ID NO.2;

[0041] SEQ ID NO.2: QPYQPVQPHQPMQPQTKREEVD

Embodiment 3

[0042] Embodiment 3: Preparation of SEQIDNO.1 polypeptide

[0043] 1. Select Fmoc-Gly(Trt)-WangResin as the resin (carrier);

[0044] 2. Fully swell the resin with DCM;

[0045] 3. Use an appropriate concentration of DBLK (hexahydropyridine + DMF) to remove the Fmoc-protecting group;

[0046] 4. Wash several times with DMF to remove DBLK;

[0047] 5. Weigh the appropriate condensing agent and activator (HBTU, NMM) and the second Fmoc-protected amino acid (Fomc-Val-OH) at the C-terminal for coupling;

[0048] 6. The ninhydrin detection method is used for detection to ensure that the connection is relatively complete;

[0049] 7. Wash several times with DMF to wash away the remaining residues and activator condensation agent;

[0050] 8. Coupling is performed according to the amino acid sequence of SEQ ID NO.1, and the method refers to steps 3-7;

[0051] 9. After connecting all the amino acids, use steps 3 and 4 to remove the final Fmoc-protecting group;

[0052] 10. Clea...

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Abstract

The invention discloses anti-caries enamel matrix protein functional polypeptide and a preparation method and application thereof. The anti-caries polypeptide comprises amino acid sequences as shown in SEQ IDNO.1 or SEQ IDNO.2 and salts or esters of the amino acid sequences. The polypeptide mainly comprises an amino acid sequence of 'QPX' inducing remineralization of early caries and further can comprise an amino acid sequence 'TKREEVD' which can act with calcium ions to induce HA nucleation. The polypeptide is low in molecular weight and has the advantages that the polypeptide can be better adsorbed by the surface of tooth enamel so as to promote remineralization of early caries; the polypeptide is convenient to synthesize, economical, effective and safe and reliable.

Description

technical field [0001] The invention relates to an anti-caries technology, in particular to an anti-caries enamel matrix protein functional polypeptide and its preparation method and application. Background technique [0002] Caries is the most common oral disease in humans. Due to the high incidence and wide prevalence of dental caries, which seriously affects oral and general health, the World Health Organization has listed it as one of the three major non-communicable diseases that human beings should prevent and treat, second only to cardiovascular diseases and tumors. [0003] As a classic anti-caries agent, fluoride has been used in various forms over the past forty years, such as fluoridated drinking water, fluoridated milk or fluoridated table salt, topical fluoride application (fluoride toothpaste, Fluoride-containing gel, fluoride-containing mouthwash), etc., which can reduce the caries rate in the population to varying degrees, and are recognized as the most effe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C07K14/00A61K38/10A61K38/16C07K1/04C07K1/08
Inventor 张凌琳律雪苹杨阳周学东李继遥
Owner SICHUAN UNIV