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Application of DWA1 gene for controlling drought resistance of rice and leaf epidermis wax synthesis

A drought resistance, leaf epidermis technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problem of the unknown regulation process of wax synthesis in plant epidermis, and achieve the effect of improving drought resistance

Inactive Publication Date: 2015-01-28
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, overall, the synthesis and regulation of plant cuticular waxes under drought stress conditions are not well understood

Method used

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  • Application of DWA1 gene for controlling drought resistance of rice and leaf epidermis wax synthesis
  • Application of DWA1 gene for controlling drought resistance of rice and leaf epidermis wax synthesis
  • Application of DWA1 gene for controlling drought resistance of rice and leaf epidermis wax synthesis

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Embodiment 1

[0021] 1. Detection of expression level of rice endogenous DWA1 gene

[0022] The applicant selected the japonica rice variety "Zhonghua 11" (or ZH11, a publicly popularized rice variety cultivated by the Institute of Crop Science, Chinese Academy of Agricultural Sciences) as the material for expression profile analysis. Rice Zhonghua 11 seedlings grown to the 4-leaf stage were subjected to various stress treatments. The drought treatment is to let it dry naturally without watering, and samples are taken after 0h, 0.5h, 1h, 4h, 8h, 12h and 24h; the low temperature stress test is to put the Zhonghua 11 (ZH11) seedlings into a 4°C artificial climate chamber (artificial climate chamber). The room is a common equipment for rice scientific research, there is no special requirement, according to the conventional light, temperature and relative humidity management), sampling after 0h, 0.5h, 1h, 4h, 8h, 12h and 24h. Abscisic acid (ABA) treatment is 100μM abscisic acid (ABA) solution ...

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Abstract

The invention belongs to the technical field of rice genetic engineering, and particularly relates to separation and clone as well as the application of a key gene DWA1 for controlling drought resistance of rice and leaf epidermis wax synthesis in the drought adverse situation in rice genetic improvement through functional verification. The nucleotide sequence of the DWA1 gene is shown in SEQ ID NO:1, and the sequence of the protein of DWA1 gene is shown in SEQ ID NO:2, and 2391 amino acids in total are coded. According to the invention, a mutant forward genetics screening technique is adopted, a drought sensitive mutant material dwa1 is separated and checked; based on the mutant, the DWA1 gene is introduced in, the drought resistance ability of the rice transgenic seedling is greatly recovered and improved, besides, the DWA1 gene in excessive expression improves the wax synthesis ability of transgenic rice, related to drought resistance ability, so that the function and application value of the gene are verified.

Description

technical field [0001] The invention belongs to the technical field of rice genetic engineering. It specifically involves the isolation, cloning and functional verification of a key gene DWA1 controlling rice drought resistance and leaf epidermis wax synthesis under drought stress, and its application in rice genetic improvement. The present invention adopts the mutant forward genetics screening method to isolate and identify a drought-sensitive mutant material dwa1. The introduction of DWA1 gene in this mutant background greatly restores and improves the drought resistance ability of rice transgenic plants, and overexpression of DWA1 gene can improve the wax synthesis ability related to drought resistance in transgenic rice, confirming the gene's function and application value. Background technique [0002] The growth and development of plants will be adversely affected by many extreme environmental factors, especially drought and water shortage will lead to large-scale p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415A01H5/00
Inventor 熊立仲朱晓义
Owner HUAZHONG AGRI UNIV