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Culture medium for gastrodia elata seed propagation germination promoting fungus third-level stain and cultivation method

A technology of sexual reproduction and cultivation method, which is applied in the field of strain cultivation, can solve the problems of complex formula of medium base material, complex formula, and interference of bacteria, so as to achieve easy acquisition, increase survival rate, and reduce the invasion of bacteria Effect

Inactive Publication Date: 2013-12-11
陕西庆瑞丰食药用菌科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a method for cultivating the third-level species of Gastrodia elata sexually reproducing germinal bacteria, which solves the problems of serious interference of bacteria and complex formulas in the cultivation process of the existing Gastrodia elata sexually reproducing germinal bacteria
[0007] Another object of the present invention is to provide a culture medium for the third-grade species of Gastrodia elata sexually propagated germination bacteria, which solves the problem of complex formula and high cost of the existing culture medium base material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The method for cultivating the third-grade species of Gastrodia elata sexually reproducing germinal bacteria comprises the following steps:

[0040] The first step is to prepare materials

[0041] Pick the leaves of Qinggang wood and dry them for later use;

[0042] Step two, soak

[0043] Place the leaves of Qinggangmu in the first step in clear water to soak for 20 hours;

[0044] The third step, drain

[0045] Pull out the leaves of Pleurotus chinensis obtained in the second step, place them in a dry place to drain, so that the moisture content in the leaves of Pleurotus chinensis is 65%;

[0046] The fourth step, mix

[0047] Mixing the leaves of C. chinensis treated in the third step with wood chips with a water content of 62% according to a mass ratio of 4:1, and stirring evenly to obtain a mixture;

[0048] Step five, bagging

[0049] The mixed material that the 4th step is made is packed in the strain bag, and loading is 80% of the strain bag capacity;

...

Embodiment 2

[0062] The method for cultivating the third-grade species of Gastrodia elata sexually reproducing germinal bacteria comprises the following steps:

[0063] The first step is to prepare materials

[0064] Pick the leaves of Qinggang wood and dry them for later use;

[0065] Step two, soak

[0066] Place the leaves of Qinggangmu in the first step in clear water to soak for 30 hours;

[0067]The third step, drain

[0068] Pull out the leaves of qinggangmu leaves obtained in the second step, place them in a dry place to drain, so that the moisture content in the leaves of qinggangmu leaves is 70%;

[0069] The fourth step, mix

[0070] It is mixed with the wood chips with water content of 68% according to the ratio of 4:1 according to the ratio of the mass ratio of the leaves of the green ganglia treated in the third step, and stirred evenly to prepare the mixture;

[0071] Step five, bagging

[0072] The mixed material that the 4th step is made is packed in the strain bag, ...

Embodiment 3

[0085] The method for cultivating the third-grade species of Gastrodia elata sexually reproducing germinal bacteria comprises the following steps:

[0086] The first step is to prepare materials

[0087] Pick the leaves of Qinggang wood and dry them for later use;

[0088] Step two, soak

[0089] Place the leaves of Qinggangmu in the first step in clear water to soak for 25 hours;

[0090] The third step, drain

[0091] Pull out the leaves of Pseudomonas chinensis prepared in the second step, place them in a dry place to drain, so that the moisture content in the leaves of Pseudomonas chinense is 68%;

[0092] The fourth step, mix

[0093] Mixing the leaves of C. chinensis treated in the third step with wood chips with a water content of 65% according to a mass ratio of 4:1, and stirring evenly to obtain a mixture;

[0094] Step five, bagging

[0095] The mixed material that the 4th step is made is packed in the strain bag, and loading is 81% of the strain bag capacity; ...

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Abstract

The invention discloses a cultivation method for a gastrodia elata seed propagation germination promoting fungus third-level stain. The method comprises ten steps of preparing, soaking, draining, stirring, bagging, covering, sterilizing, cooling, inoculating a second-level stock culture and culturing. According to the method, the problems of severe interference of infectious microbe and complex formula existing in the conventional gastrodia elata seed propagation germination promoting fungus culturing process are solved. The invention further discloses a culture medium for the gastrodia elata seed propagation germination promoting fungus third-level stain. The culture medium consists of the following raw materials: 72-74 percent of cyclobalanopsis leaves and 26-28 percent of wood flour, wherein the sum of the masses of the components is 100 percent. According to the culture medium, the wood flour supplies sufficient nutrients for the growth of the germination promoting fungus at the initial growing stage of the germination promoting fungus, so that the survival rate of the germination promoting fungus is increased; the cyclobalanopsis leaves containing enough nutrients can be used for supplying nutrients to the growth of the germination promoting fungus continuously for a long time along with the growth of the germination promoting fungus, so that rapid growth of the germination promoting fungus is ensured; meanwhile, the cyclobalanopsis leaves are easy to obtain, and fresh cyclobalanopsis leaves contain a small quantity of infectious microbe, so that the invasion of the infectious microbe in the culturing process of the germination promoting fungus can be reduced effectively.

Description

technical field [0001] The invention belongs to the technical field of strain cultivation methods, and in particular relates to a method for cultivating a tertiary species of Gastrodia elata sexually reproducing germinal bacteria, and also relates to a medium for a tertiary species of Gastrodia elata sexually reproducing germinating bacteria. Background technique [0002] Gastrodia elata is a perennial herb distributed in most parts of my country. Its dry tuber is a commonly used and relatively expensive traditional Chinese medicine. It is mostly used clinically for headaches, dizziness, numbness of limbs, convulsions in children, epilepsy, convulsions, and tetanus. The acquisition of Gastrodia elata used to rely on wild resources. After the successful transformation of the wild into home-grown species in the 1970s, the home-grown Gastrodia elata became the main commodity source. Gastrodia elata has been rated as a vulnerable species by the International Union for Conservat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05F11/00
Inventor 杨新庆
Owner 陕西庆瑞丰食药用菌科技有限公司
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