Volatile compound methyl thiobutyrate and application thereof
A technology of methyl thiobutyrate and volatile compounds, which is applied in the field of microbial biopesticides, can solve problems such as no public reports, and achieve the effects of no pollution to the environment, low cost and excellent effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0020] An experiment to detect the activity of the compound methyl thiobutyrate on Caenorhabditis elegans by two-grid method:
[0021] 1. Cultivation and preparation of nematodes
[0022] Caenorhabditis elegans was inoculated on oat medium (20 g of oat, 80 mL of water), and cultured at 28°C for 5-7 days. The required nematodes were washed out with the Baermann funnel method, and the nematodes were diluted to 1000 / mL with sterile distilled water as a nematode suspension.
[0023] 2. Test method
[0024] Add water agar to each of the two grids, add 1 mL of nematodes (about 1000) to the surface of the water agar in one grid, add 10 μL and 40 μL of the pure compound to the other grid, and seal with Parafilm. Place them at room temperature, and check and calculate nematode mortality under an optical microscope at 6, 12, and 24 hours respectively.
[0025] The method of identifying death: dead nematodes are stiff or in a "J" shape, and live nematodes are curled and twisted. Immo...
Embodiment 2
[0033] This example uses the same experimental agent and the same experimental method as Example 1, except that the nematode tested is replaced by Meloidogyne incognita. The experimental method for detecting the monomeric resistance of the volatile compound methyl thiobutyrate against Meloidogyne incognita by the two-grid method is as follows:
[0034] 1. Cultivation and preparation of nematodes
[0035] Tomato roots were used for the preparation of the southern root-knot nematode (Meloidogyne incognita). Take the root system of tomato plants with severe disease, wash the root system, count the number of root knots, continue soaking in water overnight, and collect the soaking liquid; soak and wash 3 times in the same way, and discard the root system. The residual liquid and soil were centrifuged at 2000r / min for 2min, the supernatant was discarded, 494g / L sucrose was added, and after mixing, the supernatant was cleaned with an 800-mesh separation sieve, and the larva and larv...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com