Microcarrier system, bucket and cryopreservation method for biological material cryopreservation

Abstract

The invention provides a miniature carrier system for cryopreservation of biological materials and application of the carrier system. The miniature carrier system is mainly composed of a cryopreservation unit comprising a carrying pole, a carrying pole sleeve and a carrying pole cylinder, wherein the front end of the carrying pole is inserted into the carrying pole sleeve to form a flat and slender carrying unit, and then the carrying pole and the carrying pole sleeve are arranged in the carrier pole cylinder together; the carrier pole cylinder is a hollow pipe with the average outer diameter of less than 8.5mm, and at least the bottom end of the hollow pipe is closed; the top end or the side wall of the carrier pole cylinder is provided with a pole loading hole, through which a locating unit can be placed in and taken out of the carrier pole cylinder, and loading protection and unloading technologies are implemented by virtue of the pole loading hole. On the basis of not influencing and even improving the use convenience of the miniature carrier system, space occupied for cryopreservation of a trace biological sample is reduced in multiples, and thus a floor space for cryopreservation is saved greatly. The miniature carrier system is especially suitable for cryopreservation of the trace biological materials such as embryos.

Description

technical field [0001] The invention relates to a micro-carrier system for cryopreservation of biological materials, in particular to a set of sample loading devices used for vitrification or other freezing of trace biological materials in the field of biology and medicine and its application. Background technique [0002] At present, there are many kinds of known cryocarrier systems, which can be divided into two types: cryopreservation tube type and cryopreservation rod type according to the main loading unit. The former is usually a cylindrical or partially cylindrical tubular container, one end of which is a blind end, and the other end is closed by a cap in the form of a screw thread or a buckle. This kind of container is usually relatively large, with a volume of more than 1 milliliter, and is mostly loaded and stored in a grid box on a layered carrier of a liquid nitrogen tank. Most of the current cell cryopreservation adopts this form; the latter is a rod-shaped dev...

AI Technical Summary

Problems solved by technology

For a large-scale assisted reproductive center, if the average monthly egg retrieval cycle exceeds 500, even if the space released by thawing embryos is taken into account, the liquid nitrogen tank required for adding frozen embryos may cost an average of Adding one will cause a large space occupation in a few years

This limits the development of an assisted reproductive facility

Therefore, the space utilization efficiency of the current micro-biological sample freezing device and its corresponding loading technology is relatively low, and it is still unable to meet the needs of large-scale sample cryopreservation and its development.

[0007] On the other hand, during the freezing or thawing operation, the aluminum alloy material of the rod holder in the liquid nitrogen vapor mist is more likely to condense the water vapor in the air and freeze than other plastic materials, which often causes the label part to be exposed to the liquid nitrogen for a certain period of time. Covered by condensed ice, causing inconvenience in operation

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