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A kind of highly active tea pigment and its extraction method and application
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An extraction method and technology of tea pigment, applied in tea extraction, application, food preparation, etc., can solve problems such as excessive use of organic solvents, low extraction rate of theabrownin, and easy contamination by Aspergillus, achieving low overall cost and effective The effect of high content and less impurities
Active Publication Date: 2015-08-26
杭州茗褐生物科技有限公司
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Problems solved by technology
[0007] The above method has the disadvantages of low extraction rate of theabrownin, high extraction cost, long preparation time, easy contamination by Aspergillus, excessive use of organic solvents, etc.
Method used
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Examples
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Comparison scheme
Effect test
Embodiment 1
[0041] A method for extracting highly active tea pigments, comprising the following steps:
[0042] A. Crosslink 200g of glucose oxidase to 100g of Fe with an average particle size of 70nm 3 o 4 , spread on the bottom of a PES plastic (PES is polyethersulfone resin) container with a diameter of 20cm and a height of 40cm, and add 200g CO 2 and 100g bulk Al 2 o 3 Form the enzyme mixture, seal and set aside;
[0043] B. Grind 1 kg of green tea to 100 mesh, add 10 liters of water, heat to boiling, leave to cool at room temperature (18-25° C.) for 1 hour and filter to form tea soup;
[0044] C. According to the ratio of adding 10g of enzyme mixture to 1kg of tea soup, put the enzyme mixture into the tea soup, and heat it in a sequence of 40°C for 2 hours and 20°C for 1 hour;
[0045] D. After 24 hours, put the magnet into the container, absorb the upper solution and filter it once;
[0046] E. Evaporate part of the water in the filtrate to obtain a crude tea pigment extract, ...
Embodiment 2
[0063] On the basis of Example 1, Fe in step A 3 o 4 The average particle size of the tea is changed to 60nm, and the tea leaves are pulverized to 80 mesh in step B.
[0064] The extract of gained carries out chemical composition analysis by routine method, and its result is the following table:
[0065] Element
Theabrownin
Theaflavins
Thearubigin
Content (%)
83％
11％
1％
[0066] The above results are average values in grams / 100 grams.
[0067] The highly active tea pigment extracted by the above method is applied to fruit juice drinks and dairy products, and the specific application method is as in Example 1.
Embodiment 4
[0069] On the basis of Example 1, the weight ratio of tea soup and enzyme mixture in step C was changed to 200:1, that is, the ratio of adding 10 g of enzyme mixture to 2 kg of tea soup.
[0070] The extract of gained carries out chemical composition analysis by routine method, and its result is the following table:
[0071] Element
Theabrownin
Theaflavins
Thearubigin
[0072] Content (%)
71％
4％
1％
[0073] The above results are average values in grams / 100 grams.
[0074] The highly active tea pigment extracted by the above method is applied to fruit juice drinks and dairy products, and the specific application method is as in Example 1.
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Abstract
The invention relates to a high-activity tea pigment, and an extraction method and application thereof. The high-activity tea pigment comprises 50-90% of theabrownin, 7-30% of theaflavin and 3-20% of thearubigin. The extraction method comprises the following steps: A. crosslinking glucose oxidase to nano Fe3O4, and adding CO2 and inert material Al2O3 to form an enzyme mixture; B. pulverizing tea, adding water, heating to a boiling state, cooling by standing, and filtering to form tea soup; C. mixing the enzyme mixture and tea soup, and carrying out circular heating-cooling; D. after carrying out circular heating-cooling for 12-48 hours, putting a magnet in the container, absorbing the supernate, and filtering; and E. evaporating the filtrate to obtain a tea pigment crude extract, dissolving in an alcohol water solution, concentrating and drying to obtain the high-activity tea pigment. The high-activity tea pigment is applicable to food. The content of the high-activity theabrownin in the extract can reach 65-90%.
Description
technical field [0001] The invention relates to a tea pigment and an extraction method thereof, in particular to a highly active tea pigment and an extraction method thereof. Background technique [0002] Tea pigment is a kind of water-soluble pigment mixture derived from the oxidation of polyphenolic compounds mainly composed of catechins in tea, including theaflavins, thearubigins and theabrownins. Studies have shown that theabrownin is the most stable and has the greatest biological activity. Specifically, theabrownin has the function of significantly inhibiting protein tyrosine phosphatase, and can be used for the treatment and prevention of type II diabetes, obesity diabetes, and obesity; It has various pharmacological functions such as anti-cancer and anti-ultraviolet radiation; it has strong activity of scavenging free radicals and has anti-oxidation effect; it has no toxic side effects and is safe and reliable to take. [0003] The existing theabrownin extraction m...
Claims
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Application Information
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