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Method for counting microcystis population cells

A technology of Microcystis cells and colony cells, which is applied to biochemical equipment and methods, and microbial measurement/inspection, etc., can solve problems such as damage to Microcystis colony cells, reduce the accuracy of Microcystis colony cells, and achieve consumption The effect of short time, high counting and simple experimental steps

Inactive Publication Date: 2014-01-01
TIANJIN AGRICULTURE COLLEGE
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Problems solved by technology

There are vortex shaking method, boiling method, titanium dioxide or ultraviolet treatment method for the counting method of relevant microcystis population cells in the field at present, yet under the action of vortex shaking method, the cells of microcystis population population can not be completely dispersed into single cells, but in boiling method, titanium dioxide or Under the action of ultraviolet treatment, the cells of the Microcystis population are damaged to varying degrees, so the above counting methods reduce the accuracy of the counting of the cells of the Microcystis population to varying degrees.

Method used

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Examples

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Embodiment 1

[0031] Example 1 Example of the method of the present invention being applied to the monitoring of Microcystis cell density in natural water bodies

[0032] Use the present invention to count the number of Microcystis cells when the Microcystis bloom broke out in the Jinhe River Basin, Tianjin City, on July 3, 2013:

[0033] (1) Use a 1L water collector to collect 1L of water samples from natural water bodies during the blooming period of Microcystis Jinhe; (2) Filter the collected Jinhe water samples through a microporous membrane with a pore size of about 0.45 μm , Microcystis samples were attached to the microporous membrane;

[0034] (3) Resuspend the Microcystis colony sample attached to the microporous membrane after filtration in a solution containing 10mL10 -3 mol / L EDTA solution in a 100mL beaker;

[0035] (4) After adding 100 small glass beads to the above 100mL beaker, stir magnetically at 1600rm / min for 90-110min; (see figure 1 )

[0036] (5) Take the microcyst...

Embodiment 2

[0042] Example 2 Example of the method of the present invention being applied to the monitoring of Microcystis cell density in cultured water

[0043] On July 5, 2013, at the Carp Fine Breed Farm of Tianjin Agricultural College, the Microcystis cells (containing Microcystis aeruginosa M. aeruginosa and fish-damaging Microcystis M. ichthyoblabe) with a density of 6.42×10 7 ind / L.

[0044] The method is the same as in Example 1, except that the microporous filter membrane with a pore size of 0.45 μm is replaced with a sieve with a pore size no greater than 2 μm, the results are shown in figure 2 .

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Abstract

The method discloses a method for counting microcystis population cells. First, a water sample of 1L is collected from natural water at the outburst acme of microcystis water blooms, then, the collected water sample is filtered through a microfiltration membrane with the pore diameter of about 0.45 microns, next, microcystis population samples attached to the microfiltration membrane after the filtration are suspended in a 100mL beaker containing 10mL of 10<-3>mol / L EDTA solution, after 100 small glass beads are added into the beaker, the solution in the beaker is stirred for 100min magnetically at 1600rm / min, totally dispersed single-cell microcystis samples are obtained and undamaged, and finally the microcystis cells are counted with a blood counting chamber. The method for counting microcystis population cells has the advantages of simplifying experimental steps, and being short in consumed time and capable of being used for accurately counting the microcystis population cells.

Description

technical field [0001] The invention belongs to the field of water body pollution control, in particular to a method for counting cells of Microcystis colony. Background technique [0002] Microcystis sp. is a species of cyanobacteria that is widely distributed in the world, and it is also the most common cyanobacteria bloom in my country's eutrophic water bodies. Microcystis usually exists in single-cell or double-cell form under laboratory culture conditions, but exists in a large number of groups visible to the naked eye in natural water bodies in the wild. There are vortex shaking method, boiling method, titanium dioxide or ultraviolet treatment method for the counting method of relevant microcystis population cells in the field at present, yet under the action of vortex shaking method, the cells of microcystis population population can not be completely dispersed into single cells, but in boiling method, titanium dioxide or The cells of the Microcystis population were ...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
Inventor 毕相东张树林郭永军戴伟邢克智
Owner TIANJIN AGRICULTURE COLLEGE
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