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A laser-excited cell and particle morphology and distribution measurement device

A particle shape and measurement device technology, which is applied in the fields of biomedical measurement and medical equipment, can solve the problems of reducing the detection range and accuracy of cell particle size and shape, so as to improve the use range, facilitate system debugging, and enhance electrical signal energy liquid Effect

Inactive Publication Date: 2016-03-30
JIANGXI SCI & TECH NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For flow cytometry, although forward, side scattered light and side fluorescence can be detected, cells or particles with smaller particle sizes whose back scattered light is larger than forward scattered light are not detected , thereby reducing the range and accuracy of cell size and morphology detection

Method used

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  • A laser-excited cell and particle morphology and distribution measurement device
  • A laser-excited cell and particle morphology and distribution measurement device
  • A laser-excited cell and particle morphology and distribution measurement device

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A laser-excited device for the measurement of cell and particle morphology and distribution, such as figure 1 As shown, it includes a laser radiation unit (100), a flow detection unit (200), a forward detection unit (300), a backward detection unit (400) and a side detection unit (500). like figure 2 As shown, it is characterized in that: the laser radiation unit (100) sequentially includes a laser (110), a beam expander (120), a pinhole (130), a collimating lens (140), and a focusing lens (150) along the light propagation direction. ; The flow detection unit (200) is composed of a detection chamber (210), a sheath flow tube (220), and a sample tube (230) from outside to inside, and the nozzle (240) is located below the detection chamber (210). The forward detection unit (300) is composed of a forward Fourier lens (310) and a forward scattered light detector (320). The backward detection unit (400) is composed of a backward Fourier lens (410) and a backscattered ligh...

Embodiment 2

[0038] The cells to be tested are irradiated with collimated light, such as Figure 7 shown. The optical path steps of the laser radiation unit of this scheme are as follows: the beam emitted by the laser 110 is expanded by the beam expander 120, filtered by the pinhole 130 and collimated by the collimating lens 140, then focused by the focusing lens 150, and the focused After the light passes through the field diaphragm 160 , it passes through the collimating lens 170 to collimate the light beam, and the width of the incident light beam is changed to about 100 μm before irradiating the measured cells 250 in the flow chamber 210 .

[0039] All the other are with embodiment 1.

Embodiment 3

[0041] The semiconductor laser with adjustable wavelength is adopted, and the adjustable wavelength range is 400-800nm.

[0042] All the other are with embodiment 1.

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Abstract

A fully automatic laser-excited cell and particle shape and distribution measurement device includes a laser radiation unit, a flow detection unit, a forward detection unit, a backward detection unit and a side detection unit. The device of the present invention gathers electronic technology, computer technology, laser technology, fluid technology and biomedical technology. In addition to realizing the measurement of cell size and shape, the present invention can also perform quantitative analysis on antigens and gene proteins including cytoplasm, nucleus and intracellular antigens. Using forward, backward and sideways detection methods, the range and accuracy of cell morphology and distribution measurement are greatly improved. Compared with other types of particle detectors and cell detection devices, the present invention has better functions, measurement range and accuracy. There is a greater improvement and better practicability.

Description

technical field [0001] The invention relates to the technical fields of biomedical measurement and medical equipment, in particular to a device for measuring the shape and distribution of cells and particles excited by laser. Background technique [0002] In some industrial fields, such as petrochemical, ceramics, cement, coatings, pharmaceuticals, military industry, etc., it is necessary to have a detailed understanding of particle size and distribution. At present, laser particle size analyzers are mostly used to measure particle parameters. It has the advantages of direct contact with the detection substance, but the structure of the traditional laser particle size analyzer is basically fixed, and most of them are single-beam single-lens and multi-beam multi-lens structures. For the single-beam single-lens structure, its structure is simple and the measurement range is small; for the multi-beam multi-lens structure, although the measurement range is wide, the structure is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/02G01N15/00
Inventor 任重刘国栋
Owner JIANGXI SCI & TECH NORMAL UNIV