A kind of fermentation production method of safe and efficient lactic acid bacteria product

A production method and technology of lactic acid bacteria are applied in the field of bioengineering to achieve the effects of product safety, biomass improvement and product efficiency

Active Publication Date: 2014-10-29
广州康代生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mixed fermentation can obtain some unique products, but pure fermentation is difficult to achieve

Method used

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  • A kind of fermentation production method of safe and efficient lactic acid bacteria product

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Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1 Fermentative production method of safe and efficient lactic acid bacteria product of the present invention

[0026] The fermentative production method of a kind of safe high-efficiency lactic acid bacteria product of the present invention specifically comprises the following steps:

[0027] 1. Scrape Lactobacillus casei, Lactobacillus plantarum and Pichia powder from the slant of the strains, and use drinking water to make a bacterial suspension, measure the weight of the strains in each bacterial suspension, and then press the cheese milk Bacillus, Lactobacillus plantarum and powdered Pichia pastoris are mixed in a weight ratio of 1:1:1 to prepare a bacterial suspension to obtain the original strain of mixed fermentation;

[0028] 2. Inoculate the mixed fermentation strains in the primary culture medium with an inoculum amount of 10%-15% for shaking table fermentation, control the fermentation temperature at 35-37°C, and obtain the first-class seed liquid...

Embodiment

[0030] Example 2 Fermentation production method of safe and efficient lactic acid bacteria products according to the present invention

[0031] The fermentative production method of a kind of safe high-efficiency lactic acid bacteria product of the present invention specifically comprises the following steps:

[0032] 1. Scrape Lactobacillus casei, Lactobacillus plantarum and Pichia powder from the slope of the strains, and use drinking water to make a bacterial suspension, measure the weight of the strains in each bacterial suspension, and then press the cheese milk Bacillus, Lactobacillus plantarum and powdered Pichia pastoris are mixed in a weight ratio of 2:1:1 to prepare a bacterial suspension to obtain the original strain of mixed fermentation;

[0033] 2. Inoculate the mixed fermentation strains in the primary expansion culture medium with an inoculation amount of 10%-15%, control the fermentation temperature at 35-37°C, and obtain the primary fermentation liquid afte...

Embodiment 3- Embodiment 4

[0035] Embodiment 3-embodiment 4 Fermentation production method of safe and efficient lactic acid bacteria product of the present invention

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Abstract

The invention relates to a method for producing a safe high-biomass lactobacillus product by plant-derived multi-microbe mixed fermentation, belonging to the field of bioengineering. The fermentation strain in the fermentation production method of the lactobacillus product is an associate strain composed of Lactobacillus casei, Lactobacillus plantarum and powdery Pichia pastoris. The associate strain is sequentially subjected to primary amplification culture and secondary amplification culture, and used in large-scale production of the lactobacillus product at the inoculum size of 10-15%. The method reasonably utilizes the synbiosis among the microbes, the fermentation strain used in fermentation has higher controllability, and the end product has higher biomass; and therefore, the obtained lactobacillus product is safer and more efficient when being used in animal culture, and thus, is suitable for wide application in agricultural production.

Description

technical field [0001] The invention belongs to the field of biological engineering, relates to a fermentation method of biological products, in particular to a fermentation production method of safe and efficient lactic acid bacteria products. Background technique [0002] The role of microorganisms in agriculture has been gradually recognized by people. Microbial products used in agriculture can be divided into fermented liquid and freeze-dried powder according to the form of the product. They are used in animals and aquaculture to improve the intestinal environment of animals, promote digestion and absorption, improve immunity, promote rapid growth, and reduce drug use. , to avoid veterinary drug residues and other effects; used in agricultural planting, its advantages are low cost, good application effect, no pollution to the environment, not only increase production but also improve or improve the quality of agricultural products after use, and reduce the amount of chem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/56C12R1/245C12R1/25C12R1/84
Inventor 陈明仁
Owner 广州康代生物科技有限公司
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