Novel amphiphilic ionic polypeptide and application thereof in cell culture aspect

A cell culture and amphiphilic technology, applied in animal cells, tumor/cancer cells, vertebrate cells, etc., can solve problems such as complex systems

Inactive Publication Date: 2014-01-15
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing polypeptide molecules that are gelled by changing the ionic strength are gelled under acidic conditions

Method used

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  • Novel amphiphilic ionic polypeptide and application thereof in cell culture aspect
  • Novel amphiphilic ionic polypeptide and application thereof in cell culture aspect
  • Novel amphiphilic ionic polypeptide and application thereof in cell culture aspect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: polypeptide compound ( D FEFK D Synthesis of FEFKYRGD)

[0022] Polypeptide compounds are synthesized by peptide solid-phase synthesis. Amino groups were protected using 2-chlorotrityl chloride resin and the corresponding Fmoc (fluorenylmethoxycarbonyl), tert-butyl (tert-butyl), Pbf (2,2,4,6,7-pentamethyldihydro Benzofuran-5-sulfonyl) or Boc (tert-butoxycarbonyl) protected side chain amino acids. After the first attachment from the C-terminal amino acid on the resin, the protecting group Fmoc was deprotected with 20% piperidine in anhydrous N,N'-dimethylformamide. Then the next Fmoc-protected amino acid in HBTU (benzotriazole-N,N,N',N'-tetramethyluronium hexafluorophosphate) as the coupling reagent and DIEA (diisopropylethylamine) as the catalyst Reagent conditions are attached to free amino groups. Grow the peptide chain according to the steps of the solid phase synthesis technique. After the last amino acid is attached, excess reagent is removed by...

Embodiment 2

[0023] Embodiment 2: Preparation 1 of small molecule hydrogel

[0024] Weigh 3.0 mg of the polypeptide, add 1000 μL of double distilled water to make the concentration of the polypeptide in water 0.3%, add four times the equivalent of sodium bicarbonate to adjust the pH value, and a viscous transparent liquid is formed at this time. The above solution was mixed with an equal volume of 2X PBS (phosphate buffered saline, component). The final polypeptide compound concentration was 0.15%.

[0025] The above solution gels immediately upon standing, and finally forms a transparent and uniform jelly-like small molecule hydrogel.

Embodiment 3

[0026] Embodiment 3: Preparation 2 of small molecule hydrogel

[0027] Weigh 3.0 mg of the polypeptide, add 1000 μL of double distilled water to make the concentration of the polypeptide in water 0.3%, add four times the equivalent of sodium bicarbonate to adjust the pH value, and a viscous transparent liquid is formed at this time. Mix the above solution with an equal volume of DMEM medium. The final polypeptide compound concentration was 0.15%.

[0028] The above solution gels immediately upon standing, and finally forms a transparent and uniform jelly-like small molecule hydrogel.

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Abstract

The invention relates to novel amphiphilic ionic polypeptide. A polypeptide compound has the sequence of D F E F K D F E F K Y R G D (F represents phenylalanine, D F represents D configured phenylalanine, E represents glutamic acid, K represents lysine, Y represents tyrosine, R represents arginine, G represents glycine and D represents aspartic acid). Experiments show that the amphiphilic polypeptide can quickly form small molecular hydrogel in neutral medium. Experiments verify that a three-dimensional nanometer structure formed by the hydrogel is closer to the real cell growth environment and is very suitable for the cell three-dimensional culture.

Description

technical field [0001] The invention belongs to the field of self-assembly, in particular to a small molecule hydrogel polypeptide capable of self-assembly under neutral conditions and its application in cell culture. Background technique [0002] Since the 1990s, small molecule hydrogels formed by self-assembly of polypeptides through non-covalent bonds have developed into a new type of excellent biomaterials due to their good biocompatibility, low toxicity, and biodegradability. . Small molecule hydrogels have been widely used in cell culture, tissue engineering, wound healing, regenerative medicine and drug delivery. The gelation methods of small molecule hydrogels include adjusting pH and temperature, changing ionic strength, photocatalysis, enzyme catalysis, water-soluble organic solvent assistance, ultrasound, etc. Among them, changing ionic strength, photocatalysis, and enzyme-catalyzed gelation methods are the mildest, and their biocompatibility is relatively bette...

Claims

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Application Information

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IPC IPC(8): C07K7/08C12N5/09
Inventor 杨志谋王玲冯赵骞琦史洋
Owner NANKAI UNIV
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