Application of taurine in preparing preparation for detecting or preventing myocardial ischemia injury

A technology for myocardial ischemia and acute myocardial ischemia, applied in the biological field, can solve problems such as treatment and protection of myocardial ischemia that have not been seen

Inactive Publication Date: 2014-01-15
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of taurine in preparing preparation for detecting or preventing myocardial ischemia injury
  • Application of taurine in preparing preparation for detecting or preventing myocardial ischemia injury
  • Application of taurine in preparing preparation for detecting or preventing myocardial ischemia injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1 Animal experiment

[0058] 1. Experimental materials

[0059] SD rats; TUNEL detection kit (Roche); TAUT antibody (Santa Cruz Biotechnology); bcl-2, Bax antibody (Santa Cruz Biotechnology); BSA, IHC, taurine.

[0060] 2. Experimental steps

[0061] (1) Establish an animal model of myocardial ischemia

[0062] SD rats with a body weight of 250-300g were fed conventionally; they were anesthetized by intraperitoneal injection of chloral hydrate (25mg / kg), the rats were placed in supine position, and assisted mechanical ventilation was given with a tidal volume of 180mL / min after successful tracheal intubation. The chest cavity was opened in the fifth intercostal space, and the ligation of the anterior descending coronary artery was performed to establish a myocardial ischemia model; the success of the myocardial ischemia modeling was confirmed by observing the discoloration of the anterior wall of the left ventricle and the disturbance of left ventricular ac...

Embodiment 2

[0088] Example 2 Cell experiment

[0089] 1. Experimental materials

[0090] H9C cell line; WST-8 dye was purchased from amresco company, dimethyl sulfoxide (DMSO) was purchased from merck company, high-sugar and sugar-free medium (DMEM) was purchased from invitrogen company, calf serum was purchased from Shanghai Shisheng company.

[0091] 2. Experimental steps

[0092] (1) Establish a model of H9C cell injury caused by glucose deficiency

[0093] H9C cells in logarithmic growth phase, 5×10 4 Cells per ml were inoculated in a 96-well culture plate, and cultured in 200 μl of culture medium at 37°C and 5% CO2 incubator for 12 hours; replaced with sugar-free medium to continue culture, and using WST-8 dye to detect cardiomyocytes within 3 hours of glucose deficiency Changes in cell viability to establish and identify glucose-deficiency injury models.

[0094] (2) Determination of taurine content in H9C cells damaged by glucose deficiency by HPLC

[0095] The content of tau...

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Abstract

Belonging to the biotechnology field, the invention relates to new application of taurine, in particular to application of taurine in preparing a preparation for detecting or preventing myocardial ischemia injury. The results of animal level and cellular level experiments show that during acute myocardial ischemia, the content of taurine in myocardial cells decreases, after rendering exogenous taurine, an effect against the myocardial ischemia induced cell apoptosis is generated, thus achieving the effect of protecting acute myocardial ischemia. Experiments show that taurine can serve as a detection marker of acute myocardial ischemia, pretreatment has a protective treatment effect on acute myocardial ischemia, and further can be used for preparing a preparation detecting myocardial ischemia. Experiments also show that the taurine can resist myocardial ischemia induced cell apoptosis so as to play a protection role, and further can be used for preparation of protective drugs preventing and treating acute myocardial ischemia.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a new medicinal application of taurine, in particular to the application of taurine in preparing preparations for detecting or preventing myocardial ischemia injury Background technique [0002] Myocardial ischemia is one of the common life-threatening diseases in clinical practice. The occurrence of myocardial ischemia will bring many adverse effects on the heart. The necessary energy supply is insufficient, which causes clinical manifestations such as angina pectoris, arrhythmia, and decreased heart function. Studies have found that the detection of markers of myocardial ischemia has extensive clinical application value for early diagnosis and early treatment of myocardial ischemic diseases; further, the development of drugs for the prevention and treatment of myocardial ischemia has more important clinical significance. [0003] Taurine (Tau), also known as taurine, taurine, and ta...

Claims

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Application Information

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IPC IPC(8): G01N33/48A61K31/185A61P9/10
Inventor 葛均波张悦左伋杨琳杨宇杰刘晓宇王克强
Owner FUDAN UNIV
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