A kind of biopesticide containing natural fungus to efficiently control Monochamus alternata and its application
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A technology of monochamus alternatus and natural fungi, applied in plant growth regulators, applications, biocides, etc., can solve the problems of high cost of chemical control, limitations of chemical control, ecological environment pollution, etc.
Active Publication Date: 2016-08-17
JIYANG COLLEGE OF ZHEJIANG A & F UNIV
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Problems solved by technology
But in any case, due to the uneven generation of longhorn beetles, the cost of chemical control is high and it brings serious ecological and environmental pollution problems. Therefore, chemical control has limitations.
Method used
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Embodiment 1
[0017] In 50ml corn juice and 50ml sterile water, add 0.8g beef extract, 2.7g sucrose, 7g sodium chloride, 0.8g peptone, 0.7g dipotassium hydrogen phosphate, 0.8g magnesium sulfate, stir and dissolve to make a nutrient solution; Then add 3g Beauveria bassiana oosporium, 3g Verticillium mould, 3g Paecilomyces, 1g Branchomyces, 5g Penicillium, 3g Fusarium mould, 3g Trichoderma and 4g saccharomyces in the reaction kettle, then add 71g nutrient solution , 4g of sodium alginate, mixed evenly at a constant temperature of 37°C, and finally stored at 25°C.
Embodiment 2
[0019] In 50ml corn juice and 50ml sterile water, add 0.5g beef extract, 1.6g sucrose, 6g sodium chloride, 0.6g peptone, 0.5g dipotassium hydrogen phosphate, 0.6g magnesium sulfate, stir and dissolve to make a nutrient solution; Then add 2g Beauveria bassiana, 5g Beauveria bassiana, 5g Paecilomyces, 5g Branchomyces, 5g Penicillium, 5g Fusarium, 3g Trichoderma and 2g saccharomyces in the reaction kettle, then add 58g Nutrient solution, 5g sodium alginate and 5g Tween-80, mixed evenly at a constant temperature of 25°C, and finally stored at 25°C.
Embodiment 3
[0021] In 50ml corn juice and 50ml sterile water, add 0.4g beef extract, 2.5g sucrose, 5g sodium chloride, 0.5g peptone, 0.4g dipotassium hydrogen phosphate, 0.5g magnesium sulfate, stir and dissolve evenly to make a nutrient solution; Then add 6g of Beauveria bassiana, 3g of Beauveria bassiana, 3g of Beauveria oosporium, 3g of Verticillium, 3g of Paecilomyces, 2g of Branchomyces, 2g of Penicillium, 2g of Fusarium in the reaction kettle , 1g of Trichoderma and 1g of yeast, then add 69g of nutrient solution, 5g of Tween-80, mix evenly at a constant temperature of 27°C, and finally store at 25°C.
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Abstract
The invention discloses a biological pesticide containing natural fungus for efficiently preventing and controlling Alpinia serrata and its application, belonging to the technical field of biological pesticides. It is characterized in that it contains the following substances in percentage by weight: 20-40% of natural bacteria, 50-76% of nutrient solution, and 2-15% of stabilizer. The above-mentioned biological pesticide for efficiently preventing and controlling Alpinia serrata containing natural fungus is made of natural fungus having the activity of preventing and treating Alpinia serrata as raw material, dispersing it in a nutrient solution that can keep the fungus active for a long time, and adding the biopesticide. A certain stabilizer can ensure the stability of the preparation. The prepared preparation has a good effect on the prevention and control of Alpinia serrata. It can control the pine wood nematode and reduce the pine wood nematode disease in the pine forest. It has simple processing method, sufficient raw materials, safe storage and transportation, low cost and no environmental hazards.
Description
technical field [0001] The invention belongs to the technical field of biopesticides, in particular to a biopesticide containing natural fungi for efficient control of Monochamus alternatus and its application. Background technique [0002] Monochamus monochamus is the main vector for the transmission of pine xylophilus. Japan has observed and studied Monochamus alternatus in many places since the 1960s, and has done a lot of research work on its biological characteristics, occurrence rules, the relationship between Monochamus alternata and B. xylophilus, and its control. In 1987, Tomio Kobayashi reported that Monochamus monochamus had one annual generation in Japan, and a few biennial ones in colder regions. Adults emerge from May to July, and mid-June is the eclosion period. After the eclosion adults drill out of the eclosion hole, they need to supplement nutrition for 2-3 weeks, eat the cortex of twigs less than three years old, and then mate and lay eggs. Nishizawa Kob...
Claims
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