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Applications of reagent for detecting PGRMC1 to preparation of kidney cancer diagnosis reagent and kit

A technology of reagents and uses, applied in the field of tumor molecular biology, to achieve the effect of improving survival rate, avoiding injury, and broad application prospects

Active Publication Date: 2015-06-03
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the diagnostic reagents and kits for renal cancer by detecting the expression level and phosphorylation level of PGRMC1, and there is no report on the purpose of treating renal cancer by reducing its expression level or inhibiting its phosphorylation level

Method used

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  • Applications of reagent for detecting PGRMC1 to preparation of kidney cancer diagnosis reagent and kit
  • Applications of reagent for detecting PGRMC1 to preparation of kidney cancer diagnosis reagent and kit
  • Applications of reagent for detecting PGRMC1 to preparation of kidney cancer diagnosis reagent and kit

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Embodiment 1

[0027] Embodiment 1, using the present invention to detect renal cancer

[0028] 1. Source of cell and tissue samples

[0029] Human embryonic kidney cell line HEK293 (HEK293): derived from ATCC, preserved in our laboratory; human kidney cancer cell line OS-RC-2, derived from ATCC, purchased from the Cell Bank of the Chinese Academy of Sciences;

[0030] 45 cases of kidney cancer tissues and 45 cases of normal kidney tissues adjacent to kidney cancer were provided by the Urology Department of West China Hospital of Sichuan University.

[0031] 2. Reagent source

[0032] Cell culture medium DMEM: purchased from Gibco BRL; Leu-d 3 : purchased from Isotope Laboratory, Cambridge, UK; dialyzed serum was purchased from Gibco; containing Leu-d 3 Labeled DMEM: self-made; fetal bovine serum (FBS), digestive juice trypsin (Trypsin) were purchased from Gibco BRL.

[0033]Mass spectrometry-grade trypsin Gold: purchased from Promega; acetonitrile (ACN), trifluoroacetic acid (TFA): both...

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Abstract

The invention belongs to the field of oncomolecularbiology, and particularly relates to a kidney cancer diagnosis reagent, a kit and medicaments for prevention and treatment. The kidney cancer diagnosis reagent contains a reagent capable of detecting protein expression level and / or phosphorylation level of a progesterone receptor membrane component 1. The kidney cancer diagnosis reagent provided by the invention can effectively diagnose kidney cancer diseases at the early stage, thus improving the survival rate of patients greatly. The kidney cancer diagnosis reagent provided by the invention can diagnose effectively only through less kidney cancer tissue so as to prevents hurt to the patients, and is beneficial to pertinent treatment on the patients through selecting appropriate medicaments and methods selected by doctors.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and in particular relates to a kidney cancer diagnostic reagent, kit and prevention and treatment medicine. Background technique [0002] Renal cancer is one of the common malignant tumors of the urinary system, accounting for 2-3% of all malignant tumors, accounting for about 90% of renal tumors, and its incidence rate ranks second in urinary system tumors. Kidney cancer originates from renal tubular epithelial cells, and about 75% are renal clear cell carcinomas. In recent decades, the morbidity and mortality of renal cancer have been increasing, and the incidence rate is increasing at a rate of 2% per year. The lack of effective early diagnostic markers hinders the timely diagnosis of RCC. 50% of RCC patients are diagnosed at an advanced stage, and about a quarter of RCC patients have metastasized when they are discovered, and the prognosis is poor. [0003] At present, the treatment o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574C12Q1/02
CPCC12Q1/6886C12Q2600/112G01N33/57438G01N33/94
Inventor 梁淑芳陈冰杨寒朔
Owner SICHUAN UNIV