Separation culture method of human fetal retinal pigment epithelial cells

A technology of retinal pigment and epithelial cells, which is applied to embryonic cells, animal cells, vertebrate cells, etc., can solve the problems of restricting wide application, and achieve the effect of stable cell growth, easy operation and simple steps

Inactive Publication Date: 2014-02-26
SUZHOU RUIQI BIO PHARMA CO LTD
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Problems solved by technology

The autologous transplantation of RPE cells in the subretinal space is relatively stable and has long-term curative effect,

Method used

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  • Separation culture method of human fetal retinal pigment epithelial cells
  • Separation culture method of human fetal retinal pigment epithelial cells

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Embodiment Construction

[0025] In order to enable those skilled in the art to better understand the present invention, and to make the above-mentioned advantages of the present invention more obvious and understandable, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0026] First prepare the RPE medium according to the formula in Table 1; prepare the PBS buffer, which consists of the following components: KH 2 PO 4 Concentration is 1.544118mM, NaCl concentration is 155.17241mM, Na 2 HPO 4 -7H 2 The O concentration is 2.708955 mM and the pH is 7.1-7.3. Prepare TE buffer solution, which is composed of the following components: KCl concentration is 5.333334mM, KH 2 PO 4 The concentration is 0.441176mM, NaHCO 3 Concentration is 4.166666mM, NaCl concentration is 137.93103mM, Na 2 HPO 4 -7H 2 O concentration is 0.335821mM, D-glucose concentration is 5.555555 mM, Phenol Red concentration is 0.025126mM, Na 2 -...

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Abstract

The invention provides a separation culture method of human fetal retinal pigment epithelial cells, relating to a method for separating fRPE (fetal retinal pigment epithelial) cells by utilizing a mechanical method and performing in-vitro culture. The separation culture method of the human fetal retinal pigment epithelial cells, provided by the invention, provides a new source for in-vitro culture, amplification of RPE (retinal pigment epithelial) cells and treatment of AMD (age-related macular degeneration) and other diseases with RPE injuries.

Description

technical field [0001] The invention relates to the field of biomedicine, and relates to the isolation and culture of human fetal retinal pigment epithelial cells (fRPE). Background technique [0002] RPE is a continuous monolayer of cells located outside the retina. It is named for its dice-like shape and abundant melanin in the cytoplasm. They play important functions in the maintenance and self-renewal of retinal cells, such as: phagocytosis and digestion of shed light OS of receptor cells; promote the regeneration of 11-cis-retinal, an important mediator in the visual cycle; regulate the immune response in the eye; participate in the formation of the retina-vascular barrier, etc. This abnormal physiological function of RPE cells is considered to be closely related to the occurrence of related eye diseases. For example, abnormal RPE cell barrier function can easily lead to Best vitelliform macular dystrophy (BVMD) and adult vitelliform macular dystrophy (BVMD) adult-onse...

Claims

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Application Information

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IPC IPC(8): C12N5/073
Inventor 范国平薛志刚
Owner SUZHOU RUIQI BIO PHARMA CO LTD
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