Medium for producing daunorubicin by fermenting streptomyces peucetius or streptomyces coeruleorubidus and fermentation method

Abstract

The invention relates to a medium for producing daunorubicin by fermenting streptomyces peucetius or streptomyces coeruleorubidus and a fermentation method. The medium comprises a primary seed medium, a secondary seed medium and a fermentation medium which all contain formula molasses, earthworm powder and glycerin, wherein the formula molasses comprise 0.07-0.09% of biotin, 0.02-0.05% of pantothenic acid, 0.04-0.07% of riboflavin, 0.01-0.02% of cane sugar invertase and the balance sugarcane molasses or beet molasses. By optimizing the fermentation medium formula and the fermentation technology of daunorubicin, the fermentation unit is improved, the fermentation period is shortened, the fermentation cost problem is solved, the environmental influence on sources of raw materials and auxiliary materials is furthest reduced, and the raw materials and the auxiliary materials are guaranteed to be sufficiently supplied, so that the stable efficient production of daunorubicin is realized.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a culture medium and a fermentation method for fermenting and producing daunorubicin by Streptomyces poseidoides or Streptomyces coelicolor. Background technique [0002] Daunorubicin is a secondary metabolite of Streptomyces poseidon or Streptomyces coelicolor, and its finished product is mainly used as a clinical first-line anticancer drug, and is widely used in the clinical treatment of tumors. [0003] At present, some domestic enterprises adopt traditional craft formula and three-stage fermentation mode to produce daunorubicin. The carbon sources are mainly starch and glucose, and the nitrogen sources are mainly bran and protein powder. The main problems are: [0004] 1) With different medium formulations and different compatibility ratios of carbon sources and nitrogen sources, the fermentation unit of daunorubicin is generally 1-3g / L, resulting in unstable...

AI Technical Summary

Problems solved by technology

[0004]1) The fermentation unit of daunorubicin is generally 1-3g / L due to the difference in the formula of the medium and the compatibility ratio of carbon source and nitrogen source, resulting in Unstable fermentation units

[0005]2) The fermentation period of daunorubicin is longer, with an average fermentation period of 150h

[0006]3) Combining the above factors, resulting in high energy consumption, high fermentation cost of daunorubicin, and lack of market competitiveness