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A method for improving the survival rate of bat moth larvae after taking body fluid

A technology of bat moth larvae and survival rate, which is applied in animal husbandry and other fields, can solve the problems of inability to adjust the process in time, high mortality of insects, waste of resources, etc.

Active Publication Date: 2015-10-14
YICHANG SHANCHENGSHUIDU CORDYCEPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current sampling technology puts the larvae back into the breeding box after taking the body fluid. The larvae are easily infected by bacteria or die from excessive loss of body fluid. Sometimes the larvae are even discarded after the body fluid is taken. Second or more sampling, higher worm mortality
In addition, because the evolution process of the fungus in the larvae is not clear, the process cannot be adjusted in time, which reduces the probability that the bat moth larvae will grow into Cordyceps sinensis after being infected by Chinese trichospores, resulting in a waste of resources

Method used

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  • A method for improving the survival rate of bat moth larvae after taking body fluid
  • A method for improving the survival rate of bat moth larvae after taking body fluid
  • A method for improving the survival rate of bat moth larvae after taking body fluid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 20 bat moth larvae with a size of 1.5 cm were randomly picked, each weighing 0.1 g, and divided into two groups, the control group and the test group, with 10 larvae in each group.

[0037] The larvae in the control group took 5 μL of body fluid and put them back into the breeding box directly at 15°C and 40% humidity without cold treatment.

[0038] The larvae of the test group were taken from the body fluid and placed in a refrigerator with a humidity of 82% and 4°C in the dark for 10 hours, and then transferred to a breeding box and raised under the same conditions as the control group. The volume of body fluid taken was the same as that of the control group, which was 5 μL.

[0039] After 5 days, the number of surviving larvae was observed, see Table 1.

[0040] Table 1 Larval survival rate

[0041]

[0042] The test data showed that 2 larvae survived in the control group and 9 larvae survived in the test group, and the sampling method provided by the present i...

Embodiment 2

[0044] Randomly pick 20 bat moth larvae with a size of 1.5 cm, each about 0.08 g in weight, and divide them into two groups, each with 10 larvae. Group 1 was used as the control group without taking body fluids; Group 2 was used as the test group to take 10 μL of body fluids and put them in a refrigerator with a humidity of 90% and 4°C in the dark for 6 hours, and then transferred them to a breeding box at 15°C for feeding. After 30 days, the number of surviving worms and the length of worms were observed. In group 1, 9 survived, with an average body length of 2.6 cm; in group 2, 9 survived, with an average body length of 2.4 cm. The sampling method provided by the invention has basically no influence on the growth of the larvae.

Embodiment 3

[0046] 20 bat moth larvae with a size of 1.8 cm were randomly selected, each weighing 0.13 g, and divided into two groups, each with 10 larvae. Group 1 was used as the control group without taking body fluids; Group 2 was used as the test group to take 15 μL of body fluids and put them in a refrigerator with a humidity of 80% and 2°C in the dark for 12 hours, and then transferred them to a breeding box at 15°C for feeding. After 30 days, the number of surviving worms and the length of worms were observed. In group 1, 9 survived, with an average body length of 2.9 cm; in group 2, 8 survived, with an average body length of 2.7 cm. The method provided by the invention has basically no influence on the growth of larvae.

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Abstract

The invention relates to the field of plantworms cultivation, in particular to a method for improving the survival rate of swift moth larvae with body fluid taken away. The method comprises the steps that the obtained swift moth larvae with the body fluid taken away are placed under the photophobism condition of humidity of 80-95% and temperature of 0-7DEG C for 6-20h and continue to be bred, the survival rate of the swift moth larvae with the body fluid taken away is beyond 80%, even beyond 90%, meanwhile, the same larva can be sampled many times, and bases are provided for knowing whether the host insect swift moth larvae are successfully infected by hirsutella sinensis or not and knowing the evolution process of hirsutella sinensis funguses in the infected larvae.

Description

[0001] This application claims the priority of the Chinese patent application submitted to the China Patent Office on September 20, 2012, the application number is 201210356685.4, and the title of the invention is "a method for improving the survival rate of bat moth larvae after taking body fluid", all of which The contents are incorporated by reference in this application. technical field [0002] The invention relates to the field of Cordyceps sinensis cultivation, in particular to a method for improving the survival rate of bat moth larvae after taking body fluid. Background technique [0003] Cordyceps sinensis is a famous and precious Chinese herbal medicine in China, and it is listed as the three major Chinese tonics together with ginseng and velvet antler. As early as 1757, in "Materia Medica Congxin", there is a record that "Cordyceps sinensis is sweet and flat, protects the lungs, nourishes the kidneys, nourishes the essence, stops bleeding and resolves phlegm, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
Inventor 谢俊杰郑鹏吕延华李全平李文佳朱志钢蒋均才华献春
Owner YICHANG SHANCHENGSHUIDU CORDYCEPS