Production method and application of coding suspension microchip for joint detection of multiple tumor markers

A technology for combined detection of tumor markers, applied in the field of coded suspension microchips for the joint detection of 10 tumor markers, can solve the problems of unreliable efficiency and cumbersome operation, and achieve accurate and reliable detection results, low cost, and small sample consumption Effect

Inactive Publication Date: 2014-03-26
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI +1
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Problems solved by technology

The second is the tumor marker quantitative detection kit developed b...

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  • Production method and application of coding suspension microchip for joint detection of multiple tumor markers
  • Production method and application of coding suspension microchip for joint detection of multiple tumor markers
  • Production method and application of coding suspension microchip for joint detection of multiple tumor markers

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Embodiment Construction

[0031] One aspect of the present invention aims to provide a method for preparing a coded suspension microchip that jointly detects multiple tumor markers, and its technical solution mainly includes:

[0032] providing a coded suspended microchip substrate comprising a transparent substrate and opaque planar microstructures distributed over the surface of the transparent substrate as a pattern identification code; and,

[0033] Antibody pairs corresponding to several tumor markers are provided, and one antibody in each antibody pair is connected as a probe molecule on the transparent substrate, and the other antibody is used to indicate the probe molecule and the substance to be detected. Reactive or not labeled substance attached.

[0034] In the present invention, the coded suspension microchip substrate can be prepared in various ways known in the industry, for example, one of the schemes can be:

[0035] One or more sets of planar microstructures substantially opaque to v...

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Abstract

The invention discloses a production method and application of a coding suspension microchip for joint detection of multiple tumor markers. The production method comprises the following steps: providing a coding suspension microchip base body comprising a transparent substrate and a plurality of non-transparent plane microstructures distributed on the transparent substrate and used as graphic identification codes; and providing antibody pairs corresponding to more than ten tumor markers, connecting an antibody in each antibody pair to the transparent substrate as probe molecule, and connecting the other antibody with a marking material. The coding suspension microchip can be used for detecting multiple tumor markers. The production method of the coding suspension microchip is simple and the cost is low; the coding suspension microchip can detect more than ten tumor markers in a biological sample at the same time or select several for co-check; the detection method is convenient and fast, the detection result is exact and reliable, and the specificity is high; and meanwhile, the coding suspension microchip further has the characteristics of small dosage of sample, short time and the like, and can be used for the simultaneous high-flux detection of multiple indexes in a liquid sample.

Description

technical field [0001] The invention relates to a method for detecting tumor markers, in particular to a preparation method and application of a coded suspension microchip for joint detection of 10 tumor markers. Background technique [0002] Tumor markers (tumor markers, TM) refer to the chemical substances produced by tumor tissue that can reflect the existence of the tumor itself. practical value. At present, there are many kinds of tumor markers used in clinical diagnosis, and the samples can be derived from blood, body fluid and tissue samples, etc. [0003] In the traditional way, generally only tumor markers can be checked on a specific basis, and its efficiency is very low. In view of this, the industry has developed a variety of joint detection methods for tumor markers in recent years, and the typical schemes are as follows: one is the fluorescence-encoded microsphere technology developed by Luminex Co. of the United States, the basic principle of which is to use...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57484G01N33/54306
Inventor 李炯郑克孝孔云华颜凌晨
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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