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Bacillus coagulans and its preparation method and application in biological deodorant

A technology of Bacillus coagulans and biological deodorants, applied in the application of biological deodorants, Bacillus coagulans and the field of preparation thereof, can solve problems such as no reports of Bacillus coagulans, and achieve good bacteriostatic effect, group The effect of single point and easy production control

Active Publication Date: 2016-12-07
SHENZHEN XINBAO ENVIRONMENTAL PROTECTION ENERGY SCI & TECH CO LTD
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  • Claims
  • Application Information

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Problems solved by technology

However, there has been no report on the biological deodorization of Bacillus coagulans so far

Method used

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  • Bacillus coagulans and its preparation method and application in biological deodorant
  • Bacillus coagulans and its preparation method and application in biological deodorant
  • Bacillus coagulans and its preparation method and application in biological deodorant

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Embodiment

[0039] 1. The preparation method of biological deodorant

[0040] Under aseptic operation, pick a ring of Bacillus coagulans from the preserved slant and inoculate it into the seed medium (100mL of water, 1.0-2.5g of sugar, 0.1-0.3g of yeast extract, bran filtered water 0.1, pH 5.5), cultured at 37°C for 48 hours at 200r / min to obtain seed solution.

[0041] Put the seed liquid into 10L sterilized liquid fermentation medium according to the inoculation amount of 1%-3% (water 10mL, sugar 100-250g, yeast extract 10-30g, bran filtered water 100mL, pH 5.5, 121℃ Sterilize for 30 minutes) by static culture. During the fermentation process, samples were taken every 6 hours, and the number of spores in the fermentation broth was detected by the gradient dilution plate method. When the number of spores reached 1×10 8 When the cfu / mL is reached, the fermentation is stopped to obtain the Bacillus coagulans liquid biological deodorant.

[0042] 2. Indoor simulated garbage deodorization...

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Abstract

The invention belongs to the environmental protection field, and discloses bacillus coagulans, a preparation method and an application in biological deodorants thereof. The bacillus coagulans of the invention were preserved in China General Microbiological Culture Collection Center at May 27, 2013, and has an accession number of CGMCC No. 7646. The bacterial strain of the invention is screened from excrements of livestocks, and has obvious removing effects on various offensive odor; the average removing rate of NH3 is 97.75%; the average removing rate of H2S is 97.40%; the average removing rate of total hydrocarbons is 99.85%; the average removing rate of odor concentration is 90.46%; and the average removing rate of total bacteria is 71.23%. The biological deodorant of the invention can be used for deodorization and purification of garbage, sewage, and air of livestock and poultry houses.

Description

technical field [0001] The invention belongs to the field of environmental protection, and relates to a strain of bacillus coagulans, a preparation method thereof and an application in a biological deodorant. Background technique [0002] With the acceleration of my country's urbanization process, the urban population is increasing day by day, followed by a rapid increase in the amount of urban domestic waste. According to incomplete statistics, the growth rate of garbage in my country has reached more than 10% in recent years, and nearly 150 million tons are produced every year. At present, the accumulated amount of urban domestic waste in China has reached 7 billion tons, covering an area of ​​more than 800,000 mu, of which 80% to 90% come from large and medium-sized cities. Only about 50%. More than 600 cities across the country, two-thirds of the large and medium-sized cities have fallen into the predicament of "garbage siege". Facing the dilemma of "garbage siege", t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61L9/01A61L9/012C12R1/07A61L101/52
Inventor 杨继国任杰司卫丽蓝启添
Owner SHENZHEN XINBAO ENVIRONMENTAL PROTECTION ENERGY SCI & TECH CO LTD
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