Production method of antrodia camphorata stock seeds
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A production method and the technology of the original seed are applied in the field of making the original seed of Antrodia camphorata, and can solve the problems such as the limitation of domestication and cultivation of Antrodia camphorata
Active Publication Date: 2014-04-23
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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[0005] Regarding the research on Antrodia camphorata, dozens of scholars have conducted various researches in Taiwan in the past ten years, including morphology, molecular biology, liquid fermentation, compon
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Embodiment 1
[0018] Experimental strain: Antrodia camphorata
[0019] Experimental steps:
[0020] 1. Preparation of activated slant medium:
[0021] Raw materials per liter of medium: 200 grams of potatoes (peeled), 20 grams of glucose, 20 grams of agar, 100 grams of camphor wood chips. Boil potatoes and camphor wood chips in water (20-30min), filter with gauze to obtain the filtrate, add glucose and agar to the filtrate, then make up to 1L with water, sterilize at 121°C for 30min, then pour it into a test tube, and make it 18mm×180mm test tube slant medium.
[0022] 2. Transfer tube activation of Antrodia camphorata parent species: Inoculate under sterile conditions, cut a piece of bacteria about 5mm×5mm thick about 2mm from the original Antrodia camphorata strain, transfer to the activated slant culture prepared in step 1 medium; conventional PDA medium was used as a control. There were 5 replicates for the two media respectively.
[0023] 3. Bacteria cultivation record: place it i...
Embodiment 2
[0035] Experimental strain: Antrodia camphorata
[0036] Experimental steps:
[0037] 1. Preparation of activated slant medium:
[0038] Raw materials per liter of medium: 200 grams of potatoes (peeled), 20 grams of glucose, 20 grams of agar, 100 grams of camphor wood chips. Boil potatoes and camphor wood chips in water (20-30min), filter with gauze to obtain the filtrate, add glucose and agar to the filtrate, then make up to 1L with water, sterilize at 121°C for 30min, then pour it into a test tube, and make it 18mm×180mm test tube slant medium.
[0039] 2. Transfer tube activation of Antrodia camphorata parent species: Inoculate under sterile conditions, cut a piece of bacteria about 5mm×5mm thick about 2mm from the original Antrodia camphorata strain, transfer to the activated slant culture prepared in step 1 medium; conventional PDA medium was used as a control. There were 5 replicates for the two media respectively.
[0040] 3. Bacteria cultivation record: place it i...
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Abstract
The invention discloses a production method of antrodia camphorata stock seeds. The production method is characterized by comprising the steps of inoculating antrodia camphorata to an activation inclined culture medium for conventional culture and inoculating the antrodia camphorata to a stock seed culture medium for conventional culture to obtain the antrodia camphorata stock seeds, wherein Cinnamomum kanehirai chips contained in the conventional activation inclined culture medium are replaced by Cinnamomum camphora (L.) Presl. Chips in the activation inclined culture medium and Cinnamomum kanehirai chips contained in the conventional stock seed culture medium are replaced by Cinnamomum camphora (L.) Presl. Chips in the stock seed culture medium. According to the production method of the antrodia camphorata stock seeds, the Cinnamomum kanehirai is replaced by the common Cinnamomum camphora (L.) Presl. to produce the antrodia camphorata stock seeds and accordingly a new matrix is explored for the artificial cultivation of the antrodia camphorate to replace rare Cinnamomum kanehirai wood and the great significance to the artificial cultivation of the antrodia camphorate and the protection of Cinnamomum kanehirai resouces is achieved.
Description
Technical field: [0001] The invention belongs to the technical field of domestication and cultivation of rare medicinal fungi, and in particular relates to a preparation method of antrodia camphorata original species. Background technique: [0002] Antrodia camphorata, also known as Antrodia cinnamomea or camphor mushroom, belongs to Basidomycota, Basidiomycotina, Hymenomycetes, Aphyllophorales, Polyporaceae, thin-pore The genus Antrodia, the scientific name is [Antrodia camphorata (M.Zang&C.H.Su)Sheng H.Wu, Ryvarden&T.T.Chang], and its common Latin name is [Antrodia cinnamomea T.T.Chang&W.N.Chou ], actually the same thing with different names. Initially, it was jointly reported by mainland scholar Zang Mu and Taiwan scholar Su Qinghua in 1990, and it was a new species of Ganoderma (Ganoderma comphoratum), which was mistakenly published as a member of Ganoderma (Ganoderma) because the type specimen was contaminated with Ganoderma spores. Later, Taiwanese scholars Wu Shengh...
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IPC IPC(8): A01G1/04
Inventor 黄龙花邵满超胡惠萍刘远超曹仁润杨小兵
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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