Novel microbicide anhydride modified anti-SEVI (semen-derived enhancer of viral infection) polyclonal antibody for preventing HIV (human immunodeficiency virus) sexual transmission

Abstract

The invention provides a novel microbicide anhydride modified anti-SEVI (semen-derived enhancer of viral infection) polyclonal antibody for preventing HIV (human immunodeficiency virus) sexual transmission. The novel microbicide anhydride modified anti-SEVI polyclonal antibody comprises an anti-SEVI polyclonal antibody, wherein the anti-SEVI polyclonal antibody can be used for immunizing rabbit or other tested animals by a conventional method by synthesizing a polypeptide fragment PAP248-286 of SEVI, antiserum of anti-SEVI is collected, and IgG (immunoglobulin G) of the antibody is purified to obtain the polyclonal antibody. The anhydride modified anti-SEVI polyclonal antibody is obtained by performing anhydride modification on the antibody. The anhydride modified anti-SEVI polyclonal antibody has excellent anti-virus activity to HIV-1 pseudovirion strain, zoo virus strain and anti-HIV medicinal HIV-1 drug-resistance strain, and the anhydride modified anti-SEVI polyclonal antibody can specifically bind SEVI, so that the reinforcing effect of the SEVI in seminal fluid to HIV infection can be blocked. The antibody has the beneficial effects that a dual-functional HIV sexual transmission preventing candidate microbicide can be used for resisting the strengthening capability of SEVI to HIV infection and has high-effective HIV activity.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a novel type of microbicide that can prevent sexual transmission of HIV—acid anhydride-modified anti-SEVI polyclonal antibody. Background technique [0002] AIDS (Acquired immunodeficiency syndrome, AIDS), that is, human acquired immunodeficiency syndrome, is caused by human immunodeficiency virus (Human immunodeficiency virus, HIV) invasion of the human body, continuous replication and transmission, resulting in CD4 + The significant reduction of T cells and the occurrence of opportunistic infections and cancers seriously threaten the security of the world and the survival of human beings. As of the end of 2012, approximately 35.4 million people worldwide were infected with HIV. The prevention and treatment of AIDS in my country is also in a critical period. There are more than 700,000 HIV-infected people, mainly in Yunnan, Henan, Xinjiang and Guangdong provinces. had a huge impact....

AI Technical Summary

Problems solved by technology

But the anti-SEVI polyclonal antibody itself has no antiviral activity

The anhydride-modified anti-SEVI polyclonal antibody of the present invention solves the difficulties faced in the research and development of anionic polymer microbicides, and is expected to become an ideal microbicide

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