Novel microbicide anhydride modified anti-SEVI (semen-derived enhancer of viral infection) polyclonal antibody for preventing HIV (human immunodeficiency virus) sexual transmission

A polyclonal antibody and microbicide technology, applied in the field of biochemistry, can solve the problem of no antiviral activity and achieve the effect of preventing HIV virus infection

Inactive Publication Date: 2014-04-23
SOUTHERN MEDICAL UNIVERSITY
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the anti-SEVI polyclonal antibody itself has no antiviral activity
The anhydride-modified anti-SEVI polyclonal antibody of the present inv...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel microbicide anhydride modified anti-SEVI (semen-derived enhancer of viral infection) polyclonal antibody for preventing HIV (human immunodeficiency virus) sexual transmission
  • Novel microbicide anhydride modified anti-SEVI (semen-derived enhancer of viral infection) polyclonal antibody for preventing HIV (human immunodeficiency virus) sexual transmission
  • Novel microbicide anhydride modified anti-SEVI (semen-derived enhancer of viral infection) polyclonal antibody for preventing HIV (human immunodeficiency virus) sexual transmission

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] The present invention will be further described below in conjunction with the accompanying drawings. It should be noted that this embodiment is implemented on the premise of the technical solution of the present invention, and the detailed implementation and specific operation process are given, but the protection scope of the present invention is not limited to the following embodiments.

[0033] The present invention is a novel microbicide acid anhydride modified anti-SEVI polyclonal antibody for the prevention of HIV sexual transmission, which has an anti-SEVI polyclonal antibody, wherein the anti-SEVI polyclonal antibody is synthesized to form a polypeptide fragment PAP248-286 of SEVI amyloid fibrils, using Conventional methods immunize experimental animals such as rabbits, collect anti-SEVI antiserum, and obtain after purifying antibody IgG. The anhydride-modified rabbit SEVI polyclonal antibody is prepared by the following steps:

[0034] (1) select an acid anhydr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a novel microbicide anhydride modified anti-SEVI (semen-derived enhancer of viral infection) polyclonal antibody for preventing HIV (human immunodeficiency virus) sexual transmission. The novel microbicide anhydride modified anti-SEVI polyclonal antibody comprises an anti-SEVI polyclonal antibody, wherein the anti-SEVI polyclonal antibody can be used for immunizing rabbit or other tested animals by a conventional method by synthesizing a polypeptide fragment PAP248-286 of SEVI, antiserum of anti-SEVI is collected, and IgG (immunoglobulin G) of the antibody is purified to obtain the polyclonal antibody. The anhydride modified anti-SEVI polyclonal antibody is obtained by performing anhydride modification on the antibody. The anhydride modified anti-SEVI polyclonal antibody has excellent anti-virus activity to HIV-1 pseudovirion strain, zoo virus strain and anti-HIV medicinal HIV-1 drug-resistance strain, and the anhydride modified anti-SEVI polyclonal antibody can specifically bind SEVI, so that the reinforcing effect of the SEVI in seminal fluid to HIV infection can be blocked. The antibody has the beneficial effects that a dual-functional HIV sexual transmission preventing candidate microbicide can be used for resisting the strengthening capability of SEVI to HIV infection and has high-effective HIV activity.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a novel type of microbicide that can prevent sexual transmission of HIV—acid anhydride-modified anti-SEVI polyclonal antibody. Background technique [0002] AIDS (Acquired immunodeficiency syndrome, AIDS), that is, human acquired immunodeficiency syndrome, is caused by human immunodeficiency virus (Human immunodeficiency virus, HIV) invasion of the human body, continuous replication and transmission, resulting in CD4 + The significant reduction of T cells and the occurrence of opportunistic infections and cancers seriously threaten the security of the world and the survival of human beings. As of the end of 2012, approximately 35.4 million people worldwide were infected with HIV. The prevention and treatment of AIDS in my country is also in a critical period. There are more than 700,000 HIV-infected people, mainly in Yunnan, Henan, Xinjiang and Guangdong provinces. had a huge impact....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K47/48A61K39/395A61K9/02A61P31/18
Inventor 李琳张萱萱陈金拳裘佳寅段江曼刘叔文
Owner SOUTHERN MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap