A kind of preparation method of cross-linked chitosan/hyaluronic acid composite porous scaffold

Abstract

The invention relates to the technical field of new materials and relates to a preparation method of a crosslinked chitosan / hyaluronic acid compound porous scaffold. According to the preparation method, natural biological materials such as chitosan and hyaluronic acid are used as raw materials and an organic silane coupling agent KH-560 is used as a crosslinking agent, and the crosslinked chitosan / hyaluronic acid compound porous scaffold which is made of purely natural biological materials and is excellent in mechanical property is prepared adopting a freeze-drying method. The crosslinked chitosan / hyaluronic acid compound porous scaffold prepared by adopting the preparation method has the characteristics of high porosity, high pores strength, large pores diameter and the like, is of an open type pore structure that small pores are contained in large pores, and is suitable for planting cells on the surface of the scaffold, wherein the pore diameter is 100-300 mu m and porosity is more than 90%. Compared with a chitosan scaffold, the crosslinked chitosan / hyaluronic acid compound porous scaffold has the advantages that the mechanical property is better; proliferation and differentiation effect of the cells on the surface of the porous scaffold is better due to the addition of the organic silane coupling agent, and affinity to biological cells is better.

Description

technical field [0001] The invention belongs to the technical field of new materials, and in particular relates to a preparation method of a cross-linked chitosan / hyaluronic acid composite porous support. Background technique [0002] The emergence of tissue engineering has brought bright prospects for human health and life extension. The use of biomedical materials to prepare artificial substitutes to treat diseases can solve the problem that human organs need to be replaced due to defects or loss of function, but the materials are limited. When people put their energy into artificial tissues or organs, one of the keys is the preparation of three-dimensional porous scaffold materials for tissue engineering using cell culture. [0003] At present, porous scaffold materials for tissue engineering are mainly derived from some natural polymers and synthetic polymers. Natural polymers mainly include collagen, chitin, chitosan, hyaluronic acid, gelatin, alginate, chondroitin sul...

AI Technical Summary

Problems solved by technology

Based on the advantages of convenience, easy access, taking from nature and using in nature, taking advantage of the similarity and compatibility between natural biological materials and human tissues, many researchers have chosen natural biological materials to prepare tissue engineering scaffold materials, but natural biological materials The problem of weak mechanical strength generally exists, which requires people to choose suitable natural biomaterials for blending and compounding, and to select effective cross-linking agents to improve their mechanical properties. The cross-linking agent must also be non-cytotoxic

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