Application of dihydromyricetin in preparation of liver regeneration medicine

A technology of dihydromyricetin and liver regeneration, which is applied in the field of liver regeneration, to achieve the effect of expanding the application range and promoting the regeneration of liver cells

Inactive Publication Date: 2014-04-30
HOSPITAL AFFILIATED TO GUANDONG MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are no reports of using dihydromyricetin as a drug to relieve acute liver failure and promote liver regeneration

Method used

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  • Application of dihydromyricetin in preparation of liver regeneration medicine
  • Application of dihydromyricetin in preparation of liver regeneration medicine
  • Application of dihydromyricetin in preparation of liver regeneration medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Effect of DHM on serological indicators related to liver regeneration in mice

[0028] 1. Establishment and administration of acute liver injury mouse model

[0029] The experimental animals used in this experiment were 8-week-old C57BL / 6 male mice (purchased from Guangdong Provincial Medical Experimental Animal Center), and CCl was injected intraperitoneally at 1 mL / Kg body weight 4 (Sigma) solution (CCl 4 1:3 miscibility with corn oil) to establish a mouse model of acute liver injury.

[0030] Dihydromyricetin (DHM, Sigma) was dissolved in 0.5% carboxymethylcellulose sodium (CMC-Na, Sigma) aqueous solution to a final concentration of 200 mg / mL, and injected with CCl 4 After 1 h, the dose of 150mg / Kg body weight was administered by intragastric administration, and the mice in the control group were intragastrically administered the same volume of CMC-Na at the same time. DHM solution was intragastrically administered twice a day (once every 12 hours), fo...

Embodiment 2

[0034] Example 2 Effect of DHM on hepatocyte morphology in mice with liver injury

[0035] 1. Establishment and administration of acute liver injury mouse model

[0036] The experimental animals used in this experiment were 8-week-old C57BL / 6 male mice (purchased from Guangdong Provincial Medical Experimental Animal Center), and CCl was injected intraperitoneally at 1 mL / Kg body weight 4 (Sigma) solution (CCl 4 1:3 miscibility with corn oil) to establish a mouse model of acute liver injury.

[0037] DHM (Sigma) was dissolved in 0.5% carboxymethylcellulose sodium (CMC-Na, Sigma) aqueous solution to a final concentration of 200 mg / mL, and injected with CCl 4 After 1 h, the dose of 150 mg / Kg body weight was administered by intragastric administration, and the mice in the control group were intragastrically administered the same volume of CMC-Na at the same time. DHM solution was intragastrically administered twice a day (once every 12 hours), for 7 consecutive days.

[0038...

Embodiment 3

[0041] Example 3 Effect of DHM on Hepatocyte Proliferation

[0042] Proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase δ. Because it only exists in the nucleus of proliferating cells, it is a good indicator to reflect the status of cell proliferation. This test passed the PCNA + Cell staining was used to test the effect of DHM on the proliferation ability of hepatocytes in mice with liver injury.

[0043] 1. Establishment and administration of acute liver injury mouse model

[0044] The experimental animals used in this experiment were 8-week-old C57BL / 6 male mice (purchased from Guangdong Provincial Medical Experimental Animal Center), and CCl was injected intraperitoneally at 1 mL / Kg body weight 4 (Sigma) solution (CCl 4 1:3 miscibility with corn oil) to establish a mouse model of acute liver injury.

[0045] DHM (Sigma) was dissolved in 0.5% carboxymethylcellulose sodium (CMC-Na, Sigma) aqueous solution to a final concentration of 200 ...

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PUM

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Abstract

The invention relates to the biomedical field, and particularly relates to application of dihydromyricetin in preparation of a liver treatment and regeneration promotion medicine. Dihydromyricetin shows relatively high oxidation resistance and characteristic on promoting cell growth as a flavonoid compound. The test shows that dihydromyricetin can effectively relieve liver tissue necrosis caused by acute hepatic failure and can promote hepatocyte proliferation, so as to obviously raise the survival rate of a model mouse suffering from acute hepatic failure; dihydromyricetin also can obviously improve the activity of superoxide dismutase in tissue and serum, increase the content of serum protein, and lower down the levels of glutamic oxalacetic transaminase, glutamic-pyruvic transaminase and tumor necrosis factor alpha.

Description

technical field [0001] The present invention relates to liver regeneration technology, and more specifically relates to the application of dihydromyricetin in the preparation of liver regeneration medicine. Background technique [0002] Liver failure is severe liver damage caused by a variety of factors, resulting in serious impairment or decompensation of its synthesis, detoxification, excretion, and biotransformation functions, with coagulation mechanism disorders, jaundice, hepatic encephalopathy, and ascites as the main manifestations. A group of clinical syndromes. The main causes of liver failure include hepatitis virus (mainly hepatitis B virus), liver damage caused by drugs and hepatotoxic substances, and physical injury (trauma, liver resection, etc.). [0003] Liver cancer is one of the common clinical malignant tumors. There are about 600,000 new patients with liver cancer every year in the world, and the mortality rate of patients ranks third in the world. In m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P1/16
Inventor 朱润芝李明意刘洁刘斌张庆余夏娟
Owner HOSPITAL AFFILIATED TO GUANDONG MEDICAL COLLEGE
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