SSR molecular marking method of wheat grain hardness gene

Abstract

The invention discloses a SSR molecular marking method of a wheat grain hardness gene and relates to the technical field of wheat molecular marker-assistant breeding. The SSR molecular marking method is characterized in that a PCR primer containing a microsatellite repetition section is designed in a microsatellite-feature nucleotide sequence tightly linked with a hardness gene; the target specie is subjected to PCR amplification; and then allelic variation of Pina and Pinb genes of different species is determined by polyacrylamide electrophoresis. The SSR molecular marking method has the advantages of simpleness, high speed, high stability and high allele diversity and realizes molecular marking only by common polyacrylamide electrophoresis.

Description

Technical field: [0001] The invention belongs to the technical field of wheat molecular marker assisted breeding, and relates to an SSR molecular marker method of wheat grain hardness gene. Background technique: [0002] Grain hardness is an important basis for grading and pricing domestic and foreign wheat markets. It can affect the particle size of flour, the flour extraction rate, the amount of water added for moistening wheat, and the number of damaged starch granules. Flour milled from wheat with a small hardness value has a smaller particle size, low content of damaged starch, and weak water absorption capacity, while flour milled from wheat with a large hardness value has a large particle size, high content of damaged starch, and strong water absorption capacity. Differences in these properties affect the properties of the kneaded dough and thus the type of food being prepared. For example, soft wheat is suitable for making sweet foods such as biscuits and pastries;...

AI Technical Summary

Problems solved by technology

[0004] At present, the main detection methods of SNP have the following defects: 1. Traditional detection methods based on gel electrophoresis, such as restriction fragment length polymorphism, denaturing gradient gel electrophoresis, allele-specific PCR, etc., have low detection sensitivity; 1. Nucleotide sequencing method requires an expensive 3730 sequencer, and the cost of each sample is about 10 yuan; 3. Gene chips also require expensive instruments. 4. Taqman probe method, the cost of synthesizing a fluorescent primer is 300 yuan Yuan or so