Extraction process of cordycepin (active substance) from silkworm pupa cordyceps militaris

A technology of active substances and extraction process, which is applied in the field of extraction process of active substances of traditional Chinese medicine, can solve problems such as difficult mass production, organic solvent pollution, special equipment, etc., and achieve the effects of short processing time, mild process conditions and extended storage period

Inactive Publication Date: 2014-05-07
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, solvent extraction-column chromatography purification and ultrasonic enhanced extraction-column chromatography purification methods require column loading and decolorization, which are not only troublesome to operate, but also cause pollution by toxic organic solvents; although supercritical fluid extraction-ultrasonic purification does not carry out chemical Reaction, no harmful substances are generated, but special equipment is required, and it is not easy to carry out mass production

Method used

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  • Extraction process of cordycepin (active substance) from silkworm pupa cordyceps militaris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Weigh a certain amount of polyethylene glycol 6000 and dissolve it in deionized water, prepare the solution according to Table 1, and set aside. After drying at 65°C, silkworm fungus was placed in a fully enclosed powder equipment, and crushed at 25°C by means of mechanical extrusion and impact to emulsify the solid components. The crushed silkworm grass was weighed according to the ratio in Table 1. After fully dissolving with polyethylene glycol 6000 aqueous solution, add ammonium citrate to fully shake according to the ratio in Table 1, statically separate the phases, centrifuge, remove the precipitate to obtain the supernatant; the obtained supernatant is washed with ethyl acetate After being washed with petroleum ether, vacuum-dried to obtain cordycepin.

[0016]

Embodiment 2

[0017] Embodiment 2 extraction process compares

[0018] Cordyceps is dried at 65°C, placed in a fully enclosed powder equipment, crushed at 25°C by means of mechanical extrusion and impact to emulsify the solid components, and the crushed Cordyceps is extracted and purified according to the following different methods Cordycepin.

[0019] Sample 1 (reflux extraction-column chromatography purification) (refer to Chinese patent 00130376.7 for the method): take 400 parts of silkworm fungus, add 5 times the amount of 30% ethanol (temperature 70 ° C), soak for 48 hours, and put the soaked suspension on the Reflux device, heat and reflux for 2 hours, recover ethanol, add water to precipitate, adjust pH to 3.5, filter after 24 hours, dissolve the recovered medicinal solution with water and refrigerate and precipitate at 4°C for 24 hours. Filter the cold medicinal solution with a microporous membrane (0.85 μm) and adjust the filtrate to 4.0 with 0.1mol / L for later use, put on the io...

Embodiment 3

[0025] Embodiment 3 establishes the method for measuring cordycepin content

[0026] Chromatographic conditions Chromatographic column: Knauer Europher-100C18 (5μm, 4.6×250mm); mobile phase: acetonitrile-0.05mol / LKH 2 PO 4Buffer (7.5:92.5); flow rate: 1 mL / min detection wavelength: 260 nm; column temperature: 25°C; injection volume: 10 μL.

[0027] Selection of detection wavelength Take an appropriate amount of cordycepin reference substance, add methanol to make a solution containing 0.2 mg of cordycepin per 1 mL, and perform the determination according to the above-mentioned chromatographic conditions. It can be seen from the ultraviolet spectrum of the reference substance extracted that cordycepin has the maximum absorption at 259.3nm, so the final wavelength 260nm was selected for the determination of cordycepin.

[0028] Preparation of reference substance solution Take an appropriate amount of cordycepin reference substance dried at 105°C to constant weight, weigh it ac...

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Abstract

The invention discloses a process for extracting cordycepin (active substance) from silkworm pupa cordyceps militaris. The cordycepin is a natural nucleoside new drug extracted from Cordyceps sinensis, has the activities of inhibiting tumor, resisting plasmodium, fungus and HIV-I type virus and is capable of selectively inhibiting clostridium, thereby arousing high attention in the medicine field; the cordycepin can be artificially synthesized and also can be extracted from Cordyceps sinensis, but the yield is low, the price is high, the existing purifying and extracting method is complicated in operation, and poisonous organic solvent can cause pollution, therefore, mass production is difficult to realize. A two-aqueous-phase extraction method is adopted to extract the cordycepin from the silkworm pupa cordyceps militaris, the extract content is high, the extraction efficiency is high, labor and time are saved and the process is suitable for large-scale industrial extraction.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicines, and relates to a process for extracting active substances of traditional Chinese medicines. Background technique [0002] Cordyceps militaris (Cordyc epsmilitaris), also known as Cordyceps militaris or Cordyceps militaris, is a kind of edible and medicinal fungus recognized at home and abroad. . At present, Cordyceps militaris is generally cultivated by using silkworm chrysalis as a host or rice as a medium. The National Ministry of Health has approved Cordyceps militaris as a new resource food in March 2009. Silkworm Cordyceps, also known as silkworm Cordyceps or Bombyx mori, is a combination of insects and bacteria artificially cultivated by inoculating the spores of the wild natural Cordyceps militaris (Cordyceps militaris) strain on the 5th instar silkworm. [0003] Studies have confirmed that compared with Cordyceps chrysalis, Cordyceps acid is similar, the polysaccharide of C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/16C07H1/08
CPCY02A50/30
Inventor 韦敏吕晔郑生智马红
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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