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Method for extracting Actinidia kolomikta active polyphenol and application thereof

A technology of fern and extraction method, which is applied in the fields of fern/filamentous plant medical raw materials, antidote, drug combination, etc., can solve the problems of unclear biological activity, uncontrollable quality, low purity, etc. , to achieve stable and controllable quality, reduce content, and inhibit cell apoptosis.

Active Publication Date: 2014-05-14
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It solves the problems of low purity, uncontrollable quality, and unclear biological activity in the extraction process of the active polyphenols of Pteris sylvestris, and fills the gap in the research on active polyphenols of P.

Method used

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  • Method for extracting Actinidia kolomikta active polyphenol and application thereof
  • Method for extracting Actinidia kolomikta active polyphenol and application thereof
  • Method for extracting Actinidia kolomikta active polyphenol and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A method for extracting active polyphenols from Pteris saponica, comprising the following steps:

[0034] (1) Preparation of the crude extract of the fern:

[0035] A: Methanol extraction: crush 5kg of fern rhizomes, add 30kg of methanol according to the ratio of solid to liquid of 1:6, soak at room temperature twice, 2 months / time, stir frequently during the period, filter, combine the filtrates, concentrate, the obtained Said concentration adopts vacuum concentration, vacuum degree 0.1MPa, temperature 40 ℃, obtains 190g methanol extract extract, productive rate 3.8%;

[0036] B: Petroleum ether extraction: Dissolve the obtained methanol extract extract with 2.4L distilled water to obtain a 79mg / mL methanol extract extract solution, and then extract with 1.8L petroleum ether. The petroleum ether extraction uses three extractions, specifically 800mL / First time, 600mL / Second time, 400mL / Third time, remove the fat-soluble components to obtain an aqueous solution;

[00...

Embodiment 2

[0042] A method for extracting active polyphenols from Pteris saponica, comprising the following steps:

[0043] (1) Preparation of the crude extract of the fern:

[0044] A: Methanol extraction: crush 5kg of fern rhizomes, add 25kg of methanol according to the ratio of solid to liquid of 1:5, soak at room temperature 3 times, 1 month / time, stir frequently during the period, filter, combine the filtrates, concentrate, the obtained Said concentration adopts vacuum concentration, vacuum degree 0.08MPa, temperature 45 ℃, obtains 187g methanol extract extract, productive rate 3.74%;

[0045] B: Petroleum ether extraction: Dissolve the obtained methanol extract extract with 2.64L distilled water to obtain a 71mg / mL methanol extract extract solution, and then extract with 1.98L petroleum ether. The petroleum ether extraction uses three extractions, specifically 880mL / the first time, 660mL / the second time, 440mL / the third time, remove the fat-soluble components to obtain an aq...

Embodiment 3

[0048] A method for extracting active polyphenols from Pteris saponica, comprising the following steps:

[0049] (1) Preparation of the crude extract of the fern:

[0050] A: Methanol extraction: crush 5kg of fern rhizomes, add 40kg of methanol according to the ratio of solid to liquid of 1:8, soak at room temperature twice, 2 months / time, stir frequently during the period, filter, combine the filtrates, concentrate, the obtained Said concentration adopts vacuum concentration, vacuum degree 0.09MPa, temperature 50 ℃, obtains 191g methanol extract extract, productive rate 3.82%;

[0051] B: Petroleum ether extraction: Dissolve the obtained methanol extract extract with 2.1L distilled water to obtain a 91mg / mL methanol extract extract solution, and then extract with 1.575L petroleum ether. The petroleum ether extraction uses three extractions, specifically 700mL / the first time, 525mL / the second time, 350mL / the third time, remove the fat-soluble components to obtain an aqu...

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Abstract

The invention relates to a method for extracting Actinidia kolomikta active polyphenol and application thereof. The method includes the following steps: (A): ethanol extraction (B) petroleum ether extraction; (C) n-butanol extraction; (D) silica gel column separation: dissolving the n-butanol extract obtained in step (C) in 75 g of methanol, separating with silica gel column, performing gradient elution with petroleum ether-ethyl acetate-methanol at an elution rate of 2-8 mL / min to obtain brownish-black extract; (E) Sephadex LH-20 column chromatography purification: dissolving the brownish-black extract in methanol, purifying with Sephadex LH-20 column chromatography, and performing gradient elution with CH2C12-methanol at an elution rate of 2-8 mL / min to obtain Actinidia kolomikta active polyphenol. The extraction method of the Actinidia kolomikta active polyphenol has extraction yield of 12.29%, and the obtained Actinidia kolomikta active polyphenol has purity of 95-99.9%, and has effect of resisting D-galactose induced mouse aging.

Description

technical field [0001] The invention relates to a method for extracting active polyphenols from Pteris sylvestris and an application thereof, belonging to the field of natural medicines. Background technique [0002] With the advent of the era of population aging, aging and senile diseases have become prominent problems that threaten human health. People have urgent requirements for preventing and prolonging aging. The development and application of anti-aging drugs is an important way to solve this problem. Many natural active molecules have anti-aging and life-prolonging effects, such as lycopene, resveratrol and tea polyphenols, which have been widely used in medicine, cosmetics and food industries. Natural anti-aging active substances have shown broad application prospects. [0003] Athyrium multidentatum (Doll.) Ching is a plant of the family Athyriaceae, also known as multidentatum, monkey leg vegetable, monkey leg, is a perennial fern, its fist The tender leaves and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/11A61P39/06
Inventor 盛继文
Owner WEIFANG MEDICAL UNIV
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