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Porphyromonas gingivalis culture medium for tartar specimens

A culture medium and technology of porphyrobacteria, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of inability to grow Porphyria gingivalis and low culture isolation rate, and achieve reliable detection and high isolation rate high effect

Inactive Publication Date: 2014-05-14
武改莲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are more than 500 kinds of bacteria living in the human oral cavity, among which more than 300 kinds are colonized in the subgingiva. It is precisely because of this complex sample environment that the culture and isolation rate of P. Streptococcus, Staphylococcus epidermidis, Neisseria, Lactobacillus, Spirochetes, Candida grow faster than P. gingivalis in the current medium and quickly cover the medium, P. gingivalis cannot grow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1, the formula for preparing 1000ml of medium contains: 15g of malt extract powder; 150ml of egg white liquid; 2.5ml of hemin solution with a concentration of 5mg / ml; 0.5ml of vitamin K1 ethanol solution with a concentration of 10mg / ml; Magnesium citrate 0.1g; agar 15g; cocidin 15mg; telithromycin 8mg; linezolid 10mg; asparagine 3.6g; calcium citrate tetrahydrate 0.2g; Preparation method: extract malt powder; egg protein solution; hemin solution; vitamin K1 ethanol solution; magnesium citrate; agar; cocidin; telithromycin; linezolid; asparagine; citric acid tetrahydrate Calcium was dissolved in distilled water, mixed evenly, the distilled water was adjusted to 1000ml, sterilized twice at 85°C for 45 minutes each time, and packed after sterilization.

Embodiment 2

[0017] Example 2, the formula for preparing 1000ml of medium contains: 30g of malt extract powder; 200ml of egg white liquid; 2.5ml of hemin solution with a concentration of 5mg / ml; 0.5ml of vitamin K1 ethanol solution with a concentration of 10mg / ml; Magnesium citrate 0.4g; agar 20g; coscidin 20mg; telithromycin 12mg; linezolid 5mg; asparagine 3.6g; calcium citrate tetrahydrate 0.01g; Preparation method: extract malt powder; egg protein solution; hemin solution; vitamin K1 ethanol solution; magnesium citrate; agar; cocidin; telithromycin; linezolid; asparagine; citric acid tetrahydrate Calcium was dissolved in distilled water, mixed evenly, the distilled water was adjusted to 1000ml, sterilized twice at 85°C for 45 minutes each time, and packed after sterilization.

Embodiment 3

[0018] Example 3, the formula for preparing 1000ml of medium contains: 15g of malt extract powder; 150ml of egg white liquid; 2.5ml of hemin solution with a concentration of 5mg / ml; 0.5ml of vitamin K1 ethanol solution with a concentration of 10mg / ml; Magnesium citrate 0.1g; agar 15g; cocidin 15mg; telithromycin 8mg; linezolid 10mg; asparagine 3.6g; calcium citrate tetrahydrate 0.2g; garlic squeezed juice 0.05g, L-cysteine Acid 0.1g; add distilled water to 1000ml. Preparation method: take peeled fresh garlic and wash it, press the juice with a pulverizer and filter to obtain garlic juice; take malt extract powder; egg protein solution; hemin solution; vitamin K1 ethanol solution; magnesium citrate; agar ;Cocidin; Telithromycin; Linezolid; Asparagine; Calcium citrate tetrahydrate; L-cysteine; Garlic squeezed juice was dissolved in distilled water, mixed, distilled water to 1000ml, 85 ℃ intermittent Sterilize twice, 45 minutes each time, and pack after sterilization.

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Abstract

The invention discloses a Porphyromonas gingivalis culture medium for tartar specimens and a preparation method thereof. The invention is characterized in that every 1000ml of culture medium contains malt extract, egg white, hemin solution, vitamin K1 ethanol solution, magnesium citrate, agar, synercid, telithromycin, linezolid, asparagine and the balance of distilled water. The preparation method of the optimal formula comprises the following steps: dissolving the malt extract, egg white, hemin, agar, magnesium citrate, calcium citrate tetrahydrate, synercid, garlic press juice and L-cysteine in a rhizoma polygonati boiling solution, evenly mixing, adding water to the constant volume, sterilizing and packaging. Compared with the prior art, the culture medium has the characteristics of high detection reliability and high Porphyromonas gingivalis separation rate.

Description

technical field [0001] The invention relates to the field of test microorganism culture, in particular to a culture medium for Porphyrophyllum gingivalis used for tartar specimens. Background technique [0002] Periodontitis is a common and frequently-occurring disease in the oral cavity, which belongs to bacterial infectious diseases. Epidemiological studies have shown that Porphyromonas gingivalis (P.gingivalis) is significantly related to the occurrence and development of periodontitis, and is the dominant pathogenic bacteria of periodontitis. The gingival recession and alveolar bone loss caused by periodontitis in the late stage are generally difficult to regenerate. Therefore, early diagnosis of periodontitis and early detection of P.gingivalis are very important. The current P.gingivalis detection method is mainly subgingival plaque sampling for polymerase chain reaction (polymerase chain reaction, PCR). This method requires DNA extraction and PCR primer design for sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 武改莲
Owner 武改莲
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