GBSS1 specific enzyme activity determination method
Patent Information
- Authority / Receiving Office
- CN Β· China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
- Publication Date
- 2014-05-21
- Estimated Expiration
- Not applicable Β· inactive patent
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Abstract
Description
technical field
[0001] The invention relates to the field of biotechnology; more specifically, the invention relates to a method for measuring the specific enzyme activity of GBSS 1. Background technique
[0002] GBSS1 (Granule-Bound Starch Synthase 1, 2.4.1.242) encoded by the rice waxy gene (Wx) is responsible for the synthesis of amylose in rice seed endosperm (Sano Y et al (1985). Gamma Field Symp, 24:63-77 ; Shapter et al (2009). J. Cereal Sci, 49:4-11). The expression level of rice waxy gene and the level of GBSS1 enzyme activity are significantly positively correlated with the amylose content in endosperm (Wang ZY et al(1995).The Plant Journal,7:613-622; Sano Y et al(2008 ). Theor Appl Genet, 116:979-989). Over the past 20 years, great progress has been made in the study of the expression and regulation of rice waxy genes and their application in rice quality improvement. In the above research process, the determination of GBSS1 enzyme activity is a routine experim...