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Pathotype Diagnosis Methods of Pancreatic Tumors

一种胰腺肿瘤、肿瘤的技术，应用在胰腺肿瘤的病型诊断领域，能够解决不能诊断等问题

Active Publication Date: 2016-04-20
KAGOSHIMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, even if pancreatic cancer can be detected early using the latest equipment such as PET-CT, it is often not diagnosed at a stage where it is expected to be cured, and it is a representative of refractory cancer.
In addition, even if the presence of a tumor is confirmed by imaging diagnosis, it is difficult to make a qualitative pathological diagnosis through its biopsy, etc., and it has been difficult to make a "high-grade malignant tumor that requires highly invasive surgery" or "a benign, "Observation process" judgment, which is also the characteristic of pancreaticobiliary tumors

Method used

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  • Pathotype Diagnosis Methods of Pancreatic Tumors
  • Pathotype Diagnosis Methods of Pancreatic Tumors
  • Pathotype Diagnosis Methods of Pancreatic Tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0111] [Example 1] Prediction of Pancreatic Tumor Type by Methylation Analysis of Four Mucin Genes

[0112] In this example, in human pancreatic cancer (PDAC), intraductal papillary mucinous neoplasm of gastric type (IPMN-gastric type), intraductal papillary mucinous neoplasm of intestinal type (IPMN-intestinal type) and pancreaticobiliary epithelial Intraductal papillary mucinous neoplasm of the pancreas type (IPMN-PB (pancreatobiliary)) in pancreatic juice samples by MSE method for the expression of MUC1, MUC2, MUC4 and MUC5AC gene promoters or the translational regions adjacent to the promoters involved in the expression Methylation analysis of the methylated sites (CpG sites) of the genes, and in addition, the expression of proteins encoded by these genes was confirmed by immunohistochemical staining.

[0113] 1. Materials and methods

[0114] 1-1. sample

[0115] The sample sets used in the analysis are shown in Table 1 below.

[0116] Table 1

[0117] IPM...

Embodiment 2

[0207] [Example 2] Prediction of Pancreatic Tumor Type 2 by Methylation Analysis of Four Kinds of Mucin Genes

[0208] As in Example 1, in this example, human pancreatic cancer (PDAC), gastric type intraductal papillary mucinous neoplasm of the pancreas (gastric type IPMN), intestinal type intraductal papillary mucinous neoplasm of the pancreas (intestinal type IPMN) and in pancreatic juice samples from pancreaticobiliary epithelial-type intraductal papillary mucinous neoplasms of the pancreas (P.B.-type IPMN), methylation analysis of four mucin genes (MUC1, MUC2, MUC4, and MUC5AC genes), according to the methylation Analyze the results to predict the disease type. The method of methylation analysis and disease type prediction follows the method described in Example 1.

[0209] The sample sets used in the analysis are shown in Table 10 below.

[0210] Table 10

[0211] Gastric IPMN 7 samples Intestinal IPMN 8 samples P.B.-type IPMN 5 samples PDA...

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Abstract

The object of the present invention is to provide: a method for early diagnosis of the disease type of pancreatic tumors, specifically, a detection method for determining the disease type of pancreatic tumors, the method is to detect the coding The degree of methylation of the 5' untranslated region or the region including the 5' untranslated region and the translated region of the mucin core protein gene is used as an index to classify pancreatic tumors into those selected from pancreatic cancer , Gastric-type intraductal papillary mucinous neoplasm of the pancreas, intestinal-type intraductal papillary mucinous neoplasm of the pancreas, and pancreaticobiliary epithelial-type intraductal papillary mucinous tumors of the pancreas.

Description

technical field [0001] The present invention relates to a method for diagnosing pathological types of pancreatic tumors using, for example, pancreatic juice samples. Background technique [0002] Most gastrointestinal cancers such as stomach cancer and colorectal cancer can be detected and treated at an early stage due to the development of X-ray contrast and endoscopy, and can be overcome by establishing a social system such as individual care (face down さ) or examination fees , It can be said that it is a cancer that is in the "hands of human beings". [0003] However, even with the latest equipment such as PET-CT, pancreatic cancer is often not diagnosed at a stage where it is expected to be cured even if it can be detected early, and it is a representative of refractory cancer. In addition, even if the presence of a tumor is confirmed by imaging diagnosis, it is difficult to make a qualitative pathological diagnosis through its biopsy, etc., and it has been difficult to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/09C12Q1/04C12Q1/68G01N33/50
CPCG01N33/57438C12Q2600/154C12Q2600/112C12Q2600/156C12Q1/6886
Inventor 横山势也米泽杰北本祥
Owner KAGOSHIMA UNIV
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