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Lactic acid bacterium nutrient enhancer for holothurian culture

A technology of lactic acid bacteria and enhancer, applied in application, animal feed, animal feed, etc., achieves the effects of less time-consuming, low preparation cost, and simple processing process

Inactive Publication Date: 2014-05-28
DALIAN DETONG BIO TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Seafood farming is a high-risk farming industry. Traditional small-scale seafood farming has brought huge market and technical risks to farmers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Strain recovery: Pick bacteria from a single colony of the standard strain of lactic acid bacteria and inoculate them into the lactic acid bacteria medium, and culture them at 35°C for 12 hours.

[0022] (2) Bacteria enrichment: Inoculate into lactic acid bacteria medium at a volume ratio of 4%, ferment at 35°C, and cultivate for 28 hours until the final concentration of the bacterial suspension is 10 8 cfu / g; obtained fermentation product;

[0023] Wherein, described lactic acid bacteria culture medium, its preparation method is as follows:

[0024] Take 10g of peptone, 10g of beef extract, 5g of yeast extract, 20g of glucose, 2g of diammonium citrate, 5g of sodium acetate, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate heptahydrate, 0.05 of manganese sulfate heptahydrate, and 0.25 of manganese sulfate tetrahydrate. g, Tween-80 1mL, distilled water 1000mL, sterilized at 115°C for 20min; after the medium is gradually cooled, adjust the pH of the so...

Embodiment 2

[0030] (1) Strain recovery: pick bacteria from a single colony of the standard strain of lactic acid bacteria and inoculate them into the lactic acid bacteria medium, and culture at 35°C for 12 hours;

[0031] (2) Bacteria enrichment: Inoculate into lactic acid bacteria medium at a volume ratio of 5%, ferment at 35°C, and cultivate for 48 hours until the final concentration of the bacterial suspension is 10 10 cfu / g; obtained fermentation product;

[0032] Wherein, described lactic acid bacteria culture medium, its preparation method is as follows:

[0033] Take 10g of peptone, 10g of beef extract, 5g of yeast extract, 20g of glucose, 2g of diammonium citrate, 5g of sodium acetate, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate heptahydrate, 0.05 of manganese sulfate heptahydrate, and 0.25 of manganese sulfate tetrahydrate. g, Tween-801mL, distilled water 1000mL, sterilized at 115°C for 20 minutes; after the medium is gradually cooled, adjust the pH of the so...

experiment example 3

[0039] (1) Strain recovery: Pick bacteria from a single colony of the standard strain of lactic acid bacteria and inoculate them into the lactic acid bacteria medium, and culture them at 35°C for 12 hours.

[0040] (2) Bacteria enrichment: Inoculate into lactic acid bacteria culture medium at a volume ratio of 3-5%, and carry out fermentation and cultivation at 35°C for 28 hours, until the final concentration of the bacterial suspension is 10 10 cfu / g; obtained fermentation product;

[0041] Wherein, described lactic acid bacteria culture medium, its preparation method is as follows:

[0042]Take 10g of peptone, 10g of beef extract, 5g of yeast extract, 20g of glucose, 2g of diammonium citrate, 5g of sodium acetate, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate heptahydrate, 0.05 of manganese sulfate heptahydrate, and 0.25 of manganese sulfate tetrahydrate. g, Tween-801mL, distilled water 1000mL, sterilized at 115°C for 20 minutes; after the medium is gradua...

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PUM

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Abstract

The invention discloses a lactic acid bacterium nutrient enhancer for holothurian culture. The total weight ratio of a lactic acid bacterium agent to nutrient fodder is 1:(2-10). A preparation method for the lactic acid bacterium agent comprises the following steps: collecting thalli after bacterium enrichment and washing are performed; then drying the thalli after the thalli are mixed with the nutrient fodder so as to obtain a final product with the water content of 2-5%. The lactic acid bacterium nutrient enhancer for holothurians, which is prepared through the method disclosed by the embodiment of the invention, is suitable for holothurian culture; the treating process is simple and time consumption is less; the preparation cost is lower than that of a traditional method; the powder is long in retention time and free of pollution. The lactic acid bacterium nutrient enhancer has important practical significance on holothurian culture on a large scale.

Description

technical field [0001] The invention mainly relates to the technical field of seafood disease detection, in particular to a seafood probiotic nutrition powder, in particular to a lactic acid bacteria nutrition enhancer for sea cucumber cultivation. Background technique [0002] my country has a vast sea area, superior natural conditions, and rich marine resources. But the degree of development and utilization is still very low. This also shows that my country has great potential in the development and utilization of marine resources. Liaoning sea area is an important treasure house of marine biological resources and fishery resources in China. It is rich in sea cucumbers, scallops, mussels, oysters, fish, shrimp and algae, etc., driving the development of marine economy. Strengthen multilateral and bilateral international cooperation in marine development, intensively develop and utilize sea area and island resources, rationally allocate advantageous marine industries, scie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/00A23K1/14A23K1/10A23K1/16A23K1/175
Inventor 谢克焕
Owner DALIAN DETONG BIO TECH DEV
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