A kind of biological control method of botrytis cinerea
A technology for botrytis cinerea and grapes, applied in the field of plant protection, can solve the problems of complex production process, waste of resources, multiple functions, etc., and achieve the effects of simple production method, improved sanitation, and enhanced immunity
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Embodiment 1
[0030] A biological control method for botrytis cinerea. Firstly, the blue fruit grinding liquid of snakeberry with a mass fraction of 0.4-0.6% is prepared, placed in a plastic bucket, and sealed and stored in a cool place; The ground and vines of the grape orchard are sprayed with the liquid snake berry blue fruit at least once; secondly, during the flowering period of the grapes, the grape vines are sprayed with the blue snake berry fruit liquid at least once; then, in the young fruit stage of the grapes, the grape The tree is sprayed at least once with the grinding liquid of the green fruit of the snake berry; finally, in the ripening period of the grapes, the grinding liquid of the blue fruit of the snake berry is sprayed at least once to effectively prevent and control botrytis cinerea.
Embodiment 2
[0032] Inhibition experiment of the grinding liquid of snake berry stem, leaf, green fruit and red fruit on the conidia of Botrytis cinerea.
[0033] 1. Experimental materials:
[0034] (1) Snakeberry stem grinding liquid with a mass fraction of 0.5%;
[0035] (2) Snakeberry leaf grinding liquid with a mass fraction of 0.5%;
[0036] (3) Grinding liquid of snakeberry green fruit with a mass fraction of 0.5%;
[0037] (4) Red snakeberry fruit grinding liquid with a mass fraction of 0.5%;
[0038] 2. Experimental method: Pour the experimental materials (1)-(4) into PDA medium with a diameter of 75mm, spread evenly with a triangular glass rod, and then use a hole punch with a diameter of 12mm to prepare activated ash Botrytis bacterium cake, inoculate the mycelium face down in the center of the PDA medium coated with experimental materials (1)-(4) on the surface, the relative humidity of the PDA medium is controlled at 92-97%, pH=3-5, and Placed in a constant temperature incu...
Embodiment 3
[0046] 1. Experimental time: 2013;
[0047] 2. Experimental location: a vineyard in Hefei City, Anhui Province;
[0048] 3. Experimental method:
[0049] Divide a piece of vineyard into five parts in turn, each part is three rows;
[0050] The first part does not do any treatment in the early stage; after a large number of grapes get sick, in order to reduce economic losses, the green fruit grinding liquid of snakeberry with a mass fraction of 0.5% is used for treatment;
[0051] Second part adopts the method described in embodiment 1 to process, and adopting massfraction is 0.4% snake berry cyan fruit grinding liquid, all sprays once in each period;
[0052] The third part adopts the method described in embodiment 1 to process, adopting massfraction to be 0.5% snake berry cyan fruit grinding liquid, all spraying once in each period;
[0053] The fourth part adopts the method described in embodiment 1 to process, adopting massfraction to be 0.6% snake berry cyan fruit grind...
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