Organic compound fertilizer with citrus pomace and preparation method of organic compound fertilizer
A technology of citrus peel and compound fertilizer, which is applied in the field of harmless treatment of citrus peel
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[0054] Example 1 Preparation of liquid microbial agent
[0055] (1) Preparation of Aspergillus niger CGMCC NO.7927 expansion fermentation broth
[0056] The well-preserved slant strain of Aspergillus niger CGMCC No. 7927 is activated by strains and the first, second and third liquid seeds and seed tanks are gradually expanded to obtain liquid seeds, which are connected to the fermentation tank with 6% inoculum. Cultivation temperature 30℃, stirring speed 400rpm, ventilation (V / V) 1:2, cultivation time 12h; then slowly decrease the temperature to 12℃ at a cooling rate of 2℃ / h, and incubate at a constant temperature for 18h; continue with a cooling rate of 2℃ / h Slowly lower the temperature to 3°C. At this time, add the liquid seeds to the fermentor with 4% inoculum and cultivate for 24 hours at a constant temperature; finally, slowly raise the temperature to 12°C at a heating rate of 2°C / h, and incubate at a constant temperature for 18 hours; continue at 2°C / h h The heating rate is ...
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[0070] Example 2
[0071] A preparation method of citrus peel organic compound fertilizer, including the following steps:
[0072] (1) Decreasing acidity of citrus peel
[0073] Take 50% of the total mass of the organic compound fertilizer raw materials, pulverize it to a particle size of 3mm, add 0.5% of the citrus mass acid-reducing yeast for 72h at room temperature, turn it every 12h, until the pH value of the citrus peel drops to 6;
[0074] The citrus peel is industrialized citrus waste residue after juice extraction, with a moisture content of 60% and a pH value of 3.8;
[0075] The acid-reducing yeast is composed of the expansion fermentation broth of Pichia pastoris, fission yeast and wine yeast; the yeast cell concentration in the expansion fermentation broth of Pichia pastoris, fission yeast and wine yeast is 9.0×10 respectively 8 Pcs / mL、6.0x10 8 Pcs / mL and 8.0x10 7 Pcs / mL; the mixing mass ratio of the fermentation broth of Pichia pastoris, fission yeast and wine yeast is 5:3:...
Example Embodiment
[0096] Example 3
[0097] A preparation method of citrus peel organic compound fertilizer, including the following steps:
[0098] (1) Decreasing acidity of citrus peel
[0099] Take 55% of the citrus peel residue of the total mass of the organic compound fertilizer raw materials, crush it to a particle size of 4mm, add 0.8% citrus quality acid-reducing yeast to ferment at room temperature for 36 hours, and turn it every 12h until the pH value of the citrus peel residue drops to 6;
[0100] The citrus peel is industrialized citrus waste residue after juice extraction, with a moisture content of 70% and a pH value of 3.8;
[0101] The acid-reducing yeast is composed of the expansion fermentation broth of Pichia pastoris, fission yeast and wine yeast; the yeast cell concentration in the expansion fermentation broth of Pichia pastoris, fission yeast and wine yeast is 9.0×10 respectively 8 Pcs / mL、5.0x10 8 -6.0x10 8 Pcs / mL and 7.0x10 7 -8.0x10 7 Pcs / mL; the mixing mass ratio of the fermentat...
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