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Method for preparing oyster polysaccharide

A technology of oyster polysaccharide and oyster meat, applied in the field of preparation of oyster polysaccharide

Active Publication Date: 2014-06-11
DALIAN OCEAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because oyster polysaccharide is a hydrophilic compound, so far, there is no report on the extraction of oyster polysaccharide by using a hydrophobic resin column

Method used

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  • Method for preparing oyster polysaccharide
  • Method for preparing oyster polysaccharide
  • Method for preparing oyster polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] a. Add 5 L of water to 100g oyster meat and homogenate;

[0017] b. Heat at 70°C for 60 minutes, centrifuge at 5000rpm for 30 minutes, and take the supernatant;

[0018] c. Add the supernatant to the hydrophobic resin ME-1 column for chromatography, and elute with water, 5% ethanol by mass percentage and 15% ethanol by mass percentage, until the eluent does not contain sugar (phenol-sulfuric acid method) detection), to obtain water elution fraction, 5% ethanol elution fraction and 15% ethanol elution fraction;

[0019] d. Add 80% ethanol to the water-eluted fraction, 5% ethanol-eluted fraction and 15% ethanol-eluted fraction respectively to a saturated concentration to obtain precipitates respectively. The masses of the three precipitates are 4g, 5g and 11g respectively. g.

[0020] The obtained three precipitates were passed through TSK-gel G4000PW XL Column (7.8 mm × 30 cm), the determination curve is as follows figure 2 , 3 , 4 shown. According to ...

Embodiment 2

[0022] a. Add 2 L of water to 300g oyster meat and homogenate;

[0023] b. Heat at 80°C for 50 minutes, centrifuge at 10,000 rpm for 10 minutes, and take the supernatant;

[0024] c. Add the supernatant to the hydrophobic resin ME-1 column for chromatography, and elute with water, 5% ethanol by mass percentage and 15% ethanol by mass percentage, until the eluent does not contain sugar (phenol-sulfuric acid method) detection), to obtain water elution fraction, 5% ethanol elution fraction and 15% ethanol elution fraction;

[0025] d. Add 80% ethanol to the water elution fraction, 5% ethanol elution fraction and 15% ethanol elution fraction respectively to the saturated concentration to obtain precipitates respectively. The masses of the three kinds of precipitates are 11g, 15g and 30g respectively g.

[0026] Same as Example 1, the three precipitates obtained were passed through TSK-gel G4000PW respectively XL Column (7.8 mm × 30 cm), determination curve. Accordi...

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Abstract

The invention discloses a method for preparing oyster polysaccharide. The method is simple and efficient to operate and can be used for obtaining oyster polysaccharide components of which the molecular weights are greater than 100kDa, 20-50 kDa and 2-10kDa. The method comprises the following steps in sequence: adding water into oyster meat, and homogenizing, wherein the ratio of water to oyster meat is (1-5L):(100-500g); heating the homogenate for 10-60 minutes at 50-100 DEG C, and centrifuging for 10-30 minutes at 1000-10000rpm to obtain the supernate; adding the supernate into a lyophobic resin column for chromatography, respectively eluting by using water, 5% ethanol and 15% ethanol till the eluent does not contain sugar each time, thereby respectively obtain water elution components, 5% ethanol elution components and 15% ethanol elution components; respectively adding 80% ethanol into the water elution component, the 5% ethanol elution component and the 15% ethanol elution component till saturation concentration, thus obtaining three precipitates.

Description

technical field [0001] The invention relates to a method for preparing oyster polysaccharides, in particular to a method for preparing oyster polysaccharides which is simple and efficient and can obtain oyster polysaccharide components with molecular weights greater than 100 kDa, 20-50 kDa and 2-10 kDa respectively. technical background [0002] Oyster polysaccharides have the functions of lowering blood pressure, anticoagulation, improving immune function and anti-leukopenia. Therefore, a variety of preparation methods of oyster polysaccharides have been disclosed. Generally, hot water extraction is used for rough extraction, and then acid , Alkali, ethanol, etc. as a solvent for subsequent treatment. For example, Chen Qian and other authors discussed in "Research on Oyster Glycogen (I)—Isolation, Extraction and Chemical Composition of Oyster Glycogen" published on pages 99-101 of Volume 26, Issue 6, "Food Science" in 2005. The extraction method of oyster glycogen is to pr...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 佟长青李伟曲敏金桥
Owner DALIAN OCEAN UNIV
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