Method for breeding neoseiulus californicus through apricot blossom pollen

Abstract

The invention discloses a method for breeding neoseiulus californicus through apricot blossom pollen. The method includes the steps that a mature and thick sword bean leaf is selected and washed clean and is then placed into a breeding box with the back facing upwards, and tetranychus cinnabarinus and the neoseiulus californicus are put to the leaf to be bred as an insect source; a layer of cotton is applied to the bottom of a culture dish with the diameter of 9 cm and wetted with water, and the selected thick sword bean leaf is cut into a circle and closely attached to the wet cotton to be closely surrounded by the cotton strips and is then moistened; a certain number of neoseiulus californicus eggs laid within 24 h are selected and placed on the circular leaf, the culture dish is filled with a small amount of apricot blossom pollen and is then sealed through a preservative film, the small holes are punched in the preservative film, the growth conditions of the predatory mites are observed once every 12 h, and the pollen is replaced once every 24 h; the selected eggs on the circular leaf are incubated within 24 h to 48 h and bred to 6-7 d, and mating is carried out when the mating phenomenon occurs; female mites having been mated are placed into an independent culture dish to be bred, the egg laying amount is observed once every 12 h, and laid eggs are picked out and placed into another culture dish and can also be incubated normally, feed, grow and be mated to lay eggs.

Description

technical field [0001] The invention belongs to the technical field of Neoseius californica reproduction, and in particular relates to a method for breeding Neoseius californica by using apricot pollen. Background technique [0002] Neoseius californica can prey on a variety of harmful mites such as Tetranychus cinnabarinus, E. citrus Tetranychus, and Panonychus citrus. The use of mites to control mites is of great significance for reducing pesticide residues and green agriculture. The mite was collected from Pujiang, Chengdu, Sichuan Province by Qin Guiyong, a 2009 graduate student majoring in agricultural insects and pest control at Sichuan Agricultural University. The mite was initially identified as a new domestic record species by the Xu Xuenong Research Institute of the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, and identified as a plant species. The family Phytoseiidae and the genus Neoseiulus belong to the family Phytoseiidae. [0003] ...

AI Technical Summary

Problems solved by technology

The second type is able to feed, but cannot complete subculture, that is, cannot mate and lay eggs

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