Cell culture and experiment device

Abstract

The invention discloses a cell culture and experiment device which is applied to the field of the biological and genetic engineering experiment devices. The cell culture and experiment device comprises a central distribution compartment, a culture compartment, a treatment compartment, and pipes for realizing liquid delivery between the central distribution compartment and the culture compartment and between the central distribution compartment and the treatment compartment, a distribution chamber and a piston capable of moving in the distribution chamber back and forth to change the working volume of the distribution chamber are arranged in the central distribution compartment, and a distribution valve for controlling the distribution chamber to be communicated with any pipe is arranged at the bottom end of the distribution chamber in the central distribution compartment. When in use, cells grow and reproduce in the culture compartment. A cell suspension can be delivered to the treatment compartment from the culture compartment by virtue of the distribution valve and the piston moving in the distribution chamber. Due to the design, a small device integrated with the central distribution compartment, the culture compartment and the treatment compartment is provided to take the place of manual operation while completing cell culture and various experiment items, and therefore, the time and labor are saved and the experiment raw materials are avoided being wasted.

Description

technical field [0001] The invention is used in the field of biological and genetic engineering experimental devices, and in particular relates to a cell culture and experimental device. Background technique [0002] In the field of microbiology, especially bioengineering and genetic engineering, researchers use cultured cells to perform experimental manipulations to test their theories and experiments. These basic experimental operations include but are not limited to: [0003] 1. Cell culture, especially the propagation of cells in one or more defined liquid media; [0004] 2. Measure the cell density; [0005] 3. Separate the cells from the liquid medium; [0006] 4. Resuspend the cells with fresh liquid; [0007] 5. Manipulating cells by chemical, electrical, or other physical means, such as introducing genetic material such as plasmids or oligonucleotides; [0008] 6. Use alcohol or other solutions to sterilize the instrument; [0009] 7. Rinse the instrument with...

AI Technical Summary

Problems solved by technology

When performing multiple rounds or multiple samples of experimental operations, it takes a lot of time and manpower

[0011] Although a certain degree of automation can be obtained by combining traditional instruments into small factories and adding control components, the disadvantage of this solution is that each traditional instrument is not designed for combination, and the specifications do not match each other, and the combined small factories are very difficult for It is too large for traditional biochemical laboratories, and the relatively large amount of cell culture fluid required will also make experimental raw materials too expensive

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