Traditional Chinese medicine oral liquid for preventing and treating cyanomycosis of high-quality chicken and preparation method thereof

A technology of traditional Chinese medicine oral liquid and Pseudomonas aeruginosa, which is applied in the field of Chinese veterinary medicine, can solve the problems of troublesome powder mixing, difficult absorption of traditional Chinese medicine, cumbersome procedures, etc., and achieve the effect of improving large usage, avoiding drug resistance, and easy preparation method

Inactive Publication Date: 2014-07-02
SICHUAN AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional Chinese medicine is mainly applied in powder, and the powder is cumbersome to mix, and the traditional Chinese medicine is not easy to absorb in the chicken body, the effect is slow, and the waste is large; the procedure for farmers to decoct Chinese medicine is also relatively cumbersome.

Method used

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  • Traditional Chinese medicine oral liquid for preventing and treating cyanomycosis of high-quality chicken and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 150g of catechu, 150g of black berry, 120g of Shuituanhua, 120g of heterophylla, 100g of scorpion vine, 100g of wood butterfly, 100g of peilan, 100g of Sophora japonica and 60g of licorice, grind them with a micronizer, pass through a 60-mesh sieve, Mix; then soak for 1.5 hours with 70% ethanol of 10 times the mass of medicinal materials, ultrasonically sonicate for 45 minutes, filter, then soak in 70% ethanol with 8 times the mass of medicinal materials for 1 hour, ultrasonically ultrasonically for 25 minutes, filter, combine the two filtrates, and recover Ethanol until there is no alcohol smell, add pure water to dissolve and dilute to 100% aqueous solution (according to raw medicinal materials, that is, 100mL water contains 100g traditional Chinese medicine), add activated carbon with 0.1% solution quality and boil for 10 minutes and filter (add 0.1% solution quality Tween-80 for dissolving), adjust the pH value to 7.5 with a mass percent concentration of 10% sod...

Embodiment 2

[0023] The Chinese medicine oral liquid for the prevention and treatment of high-quality poultry pseudomonas aeruginosa prepared in Example 1 was carried out in vitro antibacterial test by tube and plate method and trace broth method.

[0024] 1) In vitro susceptibility test adopts the tube-and-dish method: smear the Pseudomonas aeruginosa liquid on the MacConkey agar medium, then place a stainless steel tube on the surface of the medium, and carefully drop 1 mL of the control agent prepared in Example 1 into the steel tube with a dropper. High-quality traditional Chinese medicine oral liquid for chicken pseudomonas aeruginosa, add 1mL of berberine solution with a content of 10mg / L dropwise to the drug control well, add 1mL of normal saline to the negative control group, and then put it in a 37°C constant temperature incubator for 18-24 hours to observe the results . And measure the diameter of the inhibition zone with a vernier caliper, repeat the measurement 3 times, and tak...

Embodiment 3

[0033] 1) artificial infection treatment test: choose 600 healthy high-quality chicks and be divided into blank group, infection control group, embodiment 1 Chinese medicine oral liquid prevention group, embodiment 1 Chinese medicine oral liquid treatment group, medicine control group, every group 100, except Each chicken outside the blank group was injected with Pseudomonas aeruginosa 6×10 per 100g of body weight -6CFU / mL bacteria liquid 0.25mL, containing about 150 million bacteria. The Chinese medicine oral liquid prevention group of Example 1 was given the Chinese medicine oral liquid of Example 1 0.2 mL / piece.day 5 days in advance before exposure. Observe the clinical symptoms of chickens before and after vaccination, conduct autopsy on dead chickens, and take liver, spleen and heart for bacterial isolation and identification to determine whether the disease is successfully induced. Infection control group, embodiment 1 Chinese medicine oral liquid prevention group, embo...

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Abstract

The invention discloses a traditional Chinese medicine oral liquid for preventing and treating cyanomycosis of high-quality chicken and a preparation method thereof. The preparation method comprises the following steps: respectively pulverizing the following components in parts by weight: 15 parts of acacia catechu, 15 parts of Japanese cayratia herb, 12 parts of adina pilulifera, 12 parts of radix pseudostellariae, 10 parts of Chinese starjasmine stem, 10 parts of semen oroxyli, 10 parts of eupatorium, 10 parts of sophora flower bud and 6 parts of licorice root, in a micropulverizer; screening with a sieve of 60 meshes; and then mixing and soaking in ethyl alcohol, thereby preparing the traditional Chinese medicine oral liquid for preventing and treating cyanomycosis of high-quality chicken. The preparation method is simple; the oral liquid is convenient to take; the defects of medicine tolerance and medicine residue which are easily caused by chemical medicines are avoided; and the problems of poor absorption and large usage dosage of medicine powder in a stirring manner are solved. As a result, the oral liquid disclosed by the invention can quickly take effect.

Description

technical field [0001] The invention relates to the field of traditional Chinese veterinary medicine, in particular to a traditional Chinese medicine oral liquid for preventing and treating high-quality chicken Pseudomonas aeruginosa and a preparation method thereof. Background technique [0002] Pseudomonas aeruginosa was first isolated from wound pus by Gersard in 1882 as an conditional pathogen, which can cause human and animal infections, such as abortion of female foxes in large-scale fox farms, suppurative pneumonia in sheep flocks, Septicemia of chicks and Pseudomonas aeruginosa infection of partridges, pigeons and humans. [0003] High-quality broilers are a major feature of my country's broiler breeding industry. They come from local breeds and are suitable for the consumption requirements of local residents. They are mainly divided into yellow feathers and hemp feathers. Some areas also need chickens with jute feathers. The legs can be divided into those with green...

Claims

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Application Information

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IPC IPC(8): A61K36/87A61P31/04
Inventor 舒刚郭文竹赵小玲赵谨符华林林居纯贺常亮杨志勤殷中琼朱庆
Owner SICHUAN AGRI UNIV
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