Traditional Chinese medicine oral liquid for preventing and treating cyanomycosis of high-quality chicken and preparation method thereof
A technology of traditional Chinese medicine oral liquid and Pseudomonas aeruginosa, which is applied in the field of Chinese veterinary medicine, can solve the problems of troublesome powder mixing, difficult absorption of traditional Chinese medicine, cumbersome procedures, etc., and achieve the effect of improving large usage, avoiding drug resistance, and easy preparation method
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Embodiment 1
[0020] Take 150g of catechu, 150g of black berry, 120g of Shuituanhua, 120g of heterophylla, 100g of scorpion vine, 100g of wood butterfly, 100g of peilan, 100g of Sophora japonica and 60g of licorice, grind them with a micronizer, pass through a 60-mesh sieve, Mix; then soak for 1.5 hours with 70% ethanol of 10 times the mass of medicinal materials, ultrasonically sonicate for 45 minutes, filter, then soak in 70% ethanol with 8 times the mass of medicinal materials for 1 hour, ultrasonically ultrasonically for 25 minutes, filter, combine the two filtrates, and recover Ethanol until there is no alcohol smell, add pure water to dissolve and dilute to 100% aqueous solution (according to raw medicinal materials, that is, 100mL water contains 100g traditional Chinese medicine), add activated carbon with 0.1% solution quality and boil for 10 minutes and filter (add 0.1% solution quality Tween-80 for dissolving), adjust the pH value to 7.5 with a mass percent concentration of 10% sod...
Embodiment 2
[0023] The Chinese medicine oral liquid for the prevention and treatment of high-quality poultry pseudomonas aeruginosa prepared in Example 1 was carried out in vitro antibacterial test by tube and plate method and trace broth method.
[0024] 1) In vitro susceptibility test adopts the tube-and-dish method: smear the Pseudomonas aeruginosa liquid on the MacConkey agar medium, then place a stainless steel tube on the surface of the medium, and carefully drop 1 mL of the control agent prepared in Example 1 into the steel tube with a dropper. High-quality traditional Chinese medicine oral liquid for chicken pseudomonas aeruginosa, add 1mL of berberine solution with a content of 10mg / L dropwise to the drug control well, add 1mL of normal saline to the negative control group, and then put it in a 37°C constant temperature incubator for 18-24 hours to observe the results . And measure the diameter of the inhibition zone with a vernier caliper, repeat the measurement 3 times, and tak...
Embodiment 3
[0033] 1) artificial infection treatment test: choose 600 healthy high-quality chicks and be divided into blank group, infection control group, embodiment 1 Chinese medicine oral liquid prevention group, embodiment 1 Chinese medicine oral liquid treatment group, medicine control group, every group 100, except Each chicken outside the blank group was injected with Pseudomonas aeruginosa 6×10 per 100g of body weight -6CFU / mL bacteria liquid 0.25mL, containing about 150 million bacteria. The Chinese medicine oral liquid prevention group of Example 1 was given the Chinese medicine oral liquid of Example 1 0.2 mL / piece.day 5 days in advance before exposure. Observe the clinical symptoms of chickens before and after vaccination, conduct autopsy on dead chickens, and take liver, spleen and heart for bacterial isolation and identification to determine whether the disease is successfully induced. Infection control group, embodiment 1 Chinese medicine oral liquid prevention group, embo...
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