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A kind of biological reagent for lung cancer diagnosis, preparation method and application

A lung cancer diagnosis and biological reagent technology, applied in the fields of biology and medicine, can solve the problems of high cost, unsuitable for large-scale production, cumbersome construction process, etc., and achieve the effect of improving safety

Active Publication Date: 2018-05-29
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the construction process of the above-mentioned imaging material system is cumbersome and expensive, and is not suitable for large-scale production.

Method used

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  • A kind of biological reagent for lung cancer diagnosis, preparation method and application
  • A kind of biological reagent for lung cancer diagnosis, preparation method and application
  • A kind of biological reagent for lung cancer diagnosis, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] According to the following steps, the biological reagent for lung cancer diagnosis was prepared:

[0039] a. Amplification of OmpX gene;

[0040] The amino acid sequence of OmpX is shown in SEQ ID No.3, and the nucleotide sequence of OmpX gene is shown in SEQ ID No.4.

[0041] Primers (SEQ ID No.13 and SEQ ID No.14) were designed according to the nucleotide sequence of the OmpX gene, and PCR amplification was carried out using Escherichia coli 1061 strain as a template.

[0042] b. Obtaining the construction fragment of CPX gene;

[0043] The OmpX gene and the pBAD33 plasmid were digested with KpnI / HindIII, and the OmpX gene was transferred into the pBAD33 plasmid to obtain the pB33OpmX plasmid. Primers (SEQ ID No.15 and SEQ ID No.16) were designed to perform PCR with the pB33OpmX plasmid, The obtained product is a subsequent recombinant PCR fragment;

[0044] c. Construction of CPX gene;

[0045] The DNA sequence of CPX from the N-terminus to the C-terminus consist...

Embodiment 2

[0091] Use of the lung cancer diagnostic biological reagent prepared in Example 1 in a lung cancer diagnostic kit.

[0092] The above biological reagents for lung cancer diagnosis, that is, the fixed lung cancer-specific targeting bacteria binding experiment and the characterization experiment of the ability of the lung cancer diagnostic biological reagents to recognize xenograft tumor tissues, showed that the lung cancer diagnostic biological reagents have a lot of specificity for lung cancer A549 cells and tumor tissues The combination ability can be used to prepare a lung cancer diagnostic kit.

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Abstract

The invention discloses a lung cancer diagnostic biological reagent, which is formed by fixing a lung cancer-specific targeting bacteria by paraformaldehyde. The surface of the lung cancer-specific targeting bacteria displays a polypeptide with a sequence of SEQ ID No. 1. The lung cancer-specific targeting bacteria can express GFP protein, and the lung cancer-specific targeting bacteria is a bacterial ontology transformed into an expression vector, and the expression vector contains a specific targeting polypeptide gene and a GFP gene, The expression vector can express a specific targeting polypeptide and a GFP protein, and the sequence of the specific targeting polypeptide is SEQ ID No. 1. The present invention also provides the preparation method of the lung cancer diagnostic biological reagent and its application in the lung cancer diagnostic kit.

Description

technical field [0001] The invention relates to the fields of biology and medicine, in particular to a biological reagent for diagnosing lung cancer and its preparation method and application. Background technique [0002] Lung cancer is one of the most common and deadly malignant tumors in the world, and its incidence and growth rate ranks first among all malignant tumors. Due to the insidious onset of lung cancer, there is still a lack of effective screening and early diagnosis methods, and most patients are at an advanced stage when symptoms appear, and the prognosis is poor. In patients with early-diagnosed lung cancer, the prognosis of surgical treatment is significantly improved compared with that of advanced lung cancer, and the survival rate can reach 70%. Traditional clinical diagnostic methods such as chest radiography, bronchoscopy, sputum cytology and other methods lack sufficient specificity and sensitivity. Most lung cancer patients are diagnosed at an advance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/08C12N15/63C12N1/21G01N33/68
Inventor 朱毅敏王安欣董兵原丽华周旋濮科锋陈丽莎
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI