Human pluripotent stem cell culture medium and culture method of human pluripotent stem cells

Abstract

The invention discloses a human pluripotent stem cell culture medium and a culture method of human pluripotent stem cells. The human pluripotent stem cell culture medium comprises a basic culture medium and additives, wherein the basic culture medium is a DMEM/F12 (Dulbecco Modified Eagle Medium); the additives comprise 400-600mu g/ml sodium bicarbonate, 8-20ng/ml sodium selenate, 10-30ng/ml recombinant human insulin growth factor, 80-120ng/ml recombinant human alkali fibroblast growth factor, 7-13mu g/ml recombinant human lactoferrin, 50-70mu g/ml ascorbic acid and 1-3ng/ml recombinant human transforming growth factor. The culture medium disclosed by the invention is definite in component, stable in quality and free of animal resource components, in the culture process, no animal source cells are needed to be used as a feeder layer, cultured hES cells and hips cells are less in possibility of having human rejection reaction after being transplanted, and the growth efficiency is high.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a human pluripotent stem cell (hES / iPS) culture medium and a method for culturing the human pluripotent stem cell. Background technique [0002] In 1998, Thomson (Thomson et al., 1998) of the University of Wisconsin in the United States first successfully isolated human embryonic stem cells (hES cells) using the blastocyst inner cell mass from artificial in vitro fertilization (in vitro ferlilization, IVF). And cultivate the establishment. Cells have a high capacity for self-renewal and can expand infinitely under in vitro culture conditions. At the same time, hES cells can also differentiate into various cells derived from the three germ layers under certain conditions. Therefore, hES cells are widely used in the research of regenerative medicine, drug screening and embryonic development biology, and have been a research hotspot since its establishment. [0003] In 2006 a...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to provide a culture medium for human pluripotent stem cells (hES/iPS) and a method for culturing human pluripotent stem cells, so as to solve the problem in the prior art that human pluripotent stem cells (hES/iPS) are exposed to rich ani

Images