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The Method of Transplanting Rehmanniae Rehmanniae Detoxified Test Tube Seedlings Directly into the Soil in Net Sheds

A test-tube seedling and Rehmannia glutinosa technology, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., to achieve the effects of rapid slowing down of seedlings, shortening the time of slowing down seedlings, and simplifying the operation process

Inactive Publication Date: 2016-01-20
COTTON RES INST SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems existing in the existing methods for transplanting rehmannia root virus-free test-tube seedlings, the present invention provides a method for transplanting rehmannia root virus-free test-tube seedlings directly into the soil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Rehmannia glutinosa detoxification test tube seedlings transplanting directly into the soil using the technique of stem apex meristem culture, first using 0.10% Hgcl 2 After 8-10 minutes of disinfection, use MS+sucrose 30g / L+agar 7.0g / L+6-BA (6-benzylaminopurine) 0.25~2.0mg / L+NAA (naphthaleneacetic acid) 0.01mg / L) medium, Induce shoot tips (0.2~0.4mm) to differentiate into seedlings, and carry out rapid propagation of virus-free test tube seedlings in 1 / 4 macronutrient + 1 / 2 microelement (including vitamins, iron salts) + 1 / 2 white sugar medium. When it grows to 5 leaves, the rooted seedlings of Rehmannia glutinosa are formed. From mid-April to early May, the rooted seedlings of Rehmannia glutinosa are planted directly in the nursery, covered with soil, sprinkled with water, and timely used to build plastic film arch sheds and covered with sunshades. net, covered with shade and moisturizing for 30 days, and removed the sunshade net and plastic film.

[0014] The thickn...

Embodiment 2

[0017] Rehmannia glutinosa detoxification test tube seedlings transplanting directly into the soil using the technique of stem apex meristem culture, first using 0.10% Hgcl 2 After 8-10 minutes of disinfection, use MS+sucrose 30g / L+agar 7.0g / L+6-BA (6-benzylaminopurine) 0.25~2.0mg / L+NAA (naphthaleneacetic acid) 0.01mg / L) medium, Induce shoot tips (0.2~0.4mm) to differentiate into seedlings, and carry out rapid propagation of virus-free test tube seedlings in 1 / 4 macronutrient + 1 / 2 microelement (including vitamins, iron salts) + 1 / 2 white sugar medium. When it grows to 6 leaves, the rooted seedlings of Rehmannia glutinosa are formed. From mid-April to early May, the rooted seedlings of Rehmannia glutinosa are planted directly in the nursery, covered with soil, sprinkled with water, and timely used to build a plastic film arch shed and covered with sunshade net, covered with shade and moisturizing for 30 days, and removed the sunshade net and plastic film.

[0018] The thickne...

Embodiment 3

[0021] Rehmannia glutinosa detoxification test tube seedlings transplanting directly into the soil using the technique of stem apex meristem culture, first using 0.10% Hgcl 2 After 8-10 minutes of disinfection, use MS+sucrose 30g / L+agar 7.0g / L+6-BA (6-benzylaminopurine) 0.25~2.0mg / L+NAA (naphthaleneacetic acid) 0.01mg / L) medium, Induce shoot tips (0.2~0.4mm) to differentiate into seedlings, and carry out rapid propagation of virus-free test tube seedlings in 1 / 4 macronutrient + 1 / 2 microelement (including vitamins, iron salts) + 1 / 2 white sugar medium. When it grows to 5 leaves, the rooted seedlings of Rehmannia glutinosa are formed. From mid-April to early May, the rooted seedlings of Rehmannia glutinosa are planted directly in the nursery, covered with soil, sprinkled with water, and timely used to build plastic film arch sheds and covered with sunshades. net, covered with shade and moisturizing for 30 days, and removed the sunshade net and plastic film.

[0022] The thickn...

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Abstract

The invention relates to the technical field of transplanting test-tube seedlings of plants, in particular to a method for direct transplanting of rehmannia rehmanniae test-tube seedlings in net sheds. For the rooted seedlings of Rehmannia glutinosa, from mid-April to early May, plant the rooted seedlings of Rehmannia glutinosa directly into the nursery, cover with soil, sprinkle water, build plastic film sheds in time, and cover with sunshade nets to cover and shade Moisturize for 30 days, remove the sunshade net and plastic film. The rehmannia glutinosa detoxification test tube seedling netting shed method of the present invention directly puts into the soil for transplanting, saves the step of transplanting in a nutrient pot in the conventional method, not only simplifies the operation process, but also shortens the time for slowing down the seedlings. New leaves and new roots grow, and the average new leaf increment in 30 days is 1.85 pieces / plant. The key is that the survival rate is greatly improved, and the survival rate reaches more than 95%, which saves the cost of transplanting.

Description

technical field [0001] The invention relates to the technical field of transplanting test-tube seedlings of plants, in particular to a method for direct transplanting of rehmannia root virus-free test-tube seedlings into net sheds. Background technique [0002] Rehmannia glutinosa is a commonly used medicinal material in traditional Chinese medicine in my country. It is easily infected by a variety of viruses and pathogenic bacteria during planting, resulting in degradation of species, reduced yield, and reduced quality. Using shoot apex meristem culture technology to obtain virus-free plants is the most economical and effective way to remove virus diseases, and the survival rate of virus-free test-tube seedlings is a crucial step for the application of virus-free seedlings in production. [0003] The conventional transplanting method is to open the bottle cap of Rehmannia glutinosa and harden the seedlings in the laboratory for 2 to 3 days, then transplant them into a nutri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01H4/00
Inventor 解晓红解红娥武宗信李江辉王凌云李波陈丽
Owner COTTON RES INST SHANXI ACAD OF AGRI SCI
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