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Rehmannia virus-free test tube seedling net house direct soil entering transplanting method

A test-tube seedling and Rehmannia glutinosa technology, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., to achieve the effects of rapid slowing down of seedlings, shortening the time of slowing down seedlings, and simplifying the operation process

Inactive Publication Date: 2014-08-13
COTTON RES INST SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems existing in the existing methods for transplanting rehmannia root virus-free test-tube seedlings, the present invention provides a method for transplanting rehmannia root virus-free test-tube seedlings directly into the soil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Rehmannia glutinosa detoxification test tube seedlings transplanting directly into the soil using the technique of stem apex meristem culture, first using 0.10% Hgcl 2 After 8-10 minutes of disinfection, use MS+sucrose 30g / L+agar 7.0 g / L+6-BA (6-benzylaminopurine) 0.25~2.0mg / L+NAA (naphthaleneacetic acid) 0.01mg / L) medium, Induce shoot tips (0.2~0.4mm) to differentiate into seedlings, and carry out rapid propagation of virus-free test tube seedlings in 1 / 4 macronutrient + 1 / 2 microelement (including vitamins, iron salts) + 1 / 2 white sugar medium. When it grows to 5 leaves, the rooted seedlings of Rehmannia glutinosa are formed. From mid-April to early May, the rooted seedlings of Rehmannia glutinosa are planted directly in the nursery, covered with soil, sprinkled with water, and timely used to build plastic film arch sheds and covered with sunshades. net, covered with shade and moisturizing for 30 days, and removed the sunshade net and plastic film.

[0014] The thick...

Embodiment 2

[0017] Rehmannia glutinosa detoxification test tube seedlings transplanting directly into the soil using the technique of stem apex meristem culture, first using 0.10% Hgcl 2 After 8-10 minutes of disinfection, use MS+sucrose 30g / L+agar 7.0 g / L+6-BA (6-benzylaminopurine) 0.25~2.0mg / L+NAA (naphthaleneacetic acid) 0.01mg / L) medium, Induce shoot tips (0.2~0.4mm) to differentiate into seedlings, and carry out rapid propagation of virus-free test tube seedlings in 1 / 4 macronutrient + 1 / 2 microelement (including vitamins, iron salts) + 1 / 2 white sugar medium. When it grows to 6 leaves, the rooted seedlings of Rehmannia glutinosa are formed. From mid-April to early May, the rooted seedlings of Rehmannia glutinosa are planted directly in the nursery, covered with soil, sprinkled with water, and timely used to build a plastic film arch shed and covered with sunshade net, covered with shade and moisturizing for 30 days, and removed the sunshade net and plastic film.

[0018] The thickn...

Embodiment 3

[0021] Rehmannia glutinosa detoxification test tube seedlings transplanting directly into the soil using the technique of stem apex meristem culture, first using 0.10% Hgcl 2 After 8-10 minutes of disinfection, use MS+sucrose 30g / L+agar 7.0 g / L+6-BA (6-benzylaminopurine) 0.25~2.0mg / L+NAA (naphthaleneacetic acid) 0.01mg / L) medium, Induce shoot tips (0.2~0.4mm) to differentiate into seedlings, and carry out rapid propagation of virus-free test tube seedlings in 1 / 4 macronutrient + 1 / 2 microelement (including vitamins, iron salts) + 1 / 2 white sugar medium. When it grows to 5 leaves, the rooted seedlings of Rehmannia glutinosa are formed. From mid-April to early May, the rooted seedlings of Rehmannia glutinosa are planted directly in the nursery, covered with soil, sprinkled with water, and timely used to build a plastic film arch shed and covered with sunshade net, covered with shade and moisturizing for 30 days, and removed the sunshade net and plastic film.

[0022] The thickn...

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Abstract

The invention relates to the technical field of plant test tube seedling outside transplanting, in particular to a rehmannia virus-free test tube seedling net house direct soil entering transplanting method, which comprises the following steps that rehmannia virus-free tissue culture is carried out in a test tube, when rehmannia grows to 5 to 6 leaves, rehmannia virus-free rooting seedlings are formed, in middle April to early May, the rehmannia virus-free rooting seedlings are directly planted into tree nursery land, soil covering and watering are carried out, a plastic film arch shed is adopted and built in time, in addition, a sun shielding net is added and covered, covering shade shielding and moisture preservation are carried out for 30 days, and the sun shielding net and the plastic film are removed. The rehmannia virus-free test tube seedling net house direct soil entering transplanting method has the advantages that the nutrition pot transplanting step in the conversional method is omitted, the operation process is simplified, in addition, the seedling survival time is shortened, new leaves and new roots can grow out after 15 days of the transplanting, the average blade increasing quantity is 1.85 leaves / strain in 30 days, the survival rate is mainly and greatly improved, the survival rate reaches more than 95 percent, and the transplanting cost is reduced.

Description

technical field [0001] The invention relates to the technical field of transplanting test-tube seedlings of plants, in particular to a method for direct transplanting of rehmannia root virus-free test-tube seedlings into net sheds. Background technique [0002] Rehmannia glutinosa is a commonly used medicinal material in traditional Chinese medicine in my country. It is easily infected by a variety of viruses and pathogenic bacteria during planting, resulting in degradation of species, reduced yield, and reduced quality. Using shoot apex meristem culture technology to obtain virus-free plants is the most economical and effective way to remove virus diseases, and the survival rate of virus-free test-tube seedlings is a crucial step for the application of virus-free seedlings in production. [0003] The conventional transplanting method is to open the bottle cap of Rehmannia glutinosa and harden the seedlings in the laboratory for 2 to 3 days, then transplant them into a nutr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01H4/00
Inventor 解晓红解红娥武宗信李江辉王凌云李波陈丽
Owner COTTON RES INST SHANXI ACAD OF AGRI SCI
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