Method of direct transplanting of sweet potato virus-free test tube seedlings into net shed

A technology for test-tube seedlings and sweet potatoes, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of improving survival rate, growing robustly, and improving transplanting survival rate

Inactive Publication Date: 2016-01-20
COTTON RES INST SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems existing in the existing method for transplanting sweet potato virus-free test-tube seedlings, the present invention provides a method for directly transplanting sweet potato virus-free test-tube seedlings into the soil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The method of transplanting sweet potato virus-free test tube seedlings directly into the soil using the stem apex meristem culture technology, the sweet potato stem section seedlings were first treated with 0.10% Hgcl 2 After 8-10 minutes of disinfection, use MS+sucrose 30g / L+agar 7.0g / L+6-BA (6-benzylaminopurine) 0.25~2.0mg / L+NAA (naphthaleneacetic acid) 0.01mg / L) medium, Induce the stem tip (0.2~0.4mm) to differentiate into seedlings, and after the strains formed are tested to be virus-free by grafting of Brazilian cows, 1 / 4 macronutrients + 1 / 2 microelements (including vitamins, iron salts) + 1 / 2 white Rapid propagation of test-tube plantlets in granulated sugar medium. Grow to 5 leaves and form the rooted seedlings of sweet potato detoxification, directly plant the rooted seedlings of sweet potato detoxification into the nursery field, cover with soil, sprinkle water, adopt in time to build a plastic film shed, and add a sunshade net to cover, shade and moisturize ...

Embodiment 2

[0017] The method of transplanting sweet potato virus-free test tube seedlings directly into the soil using the stem apex meristem culture technology, the sweet potato stem section seedlings were first treated with 0.10% Hgcl 2 After 8-10 minutes of disinfection, use MS+sucrose 30g / L+agar 7.0g / L+6-BA (6-benzylaminopurine) 0.25~2.0mg / L+NAA (naphthaleneacetic acid) 0.01mg / L) medium, Induce the stem tip (0.2~0.4mm) to differentiate into seedlings, and after the strains formed are tested to be virus-free by grafting of Brazilian cows, 1 / 4 macronutrients + 1 / 2 microelements (including vitamins, iron salts) + 1 / 2 white Rapid propagation of test-tube plantlets in granulated sugar medium. Grow to 6 leaves to form the rooted seedlings of sweet potato detoxification, directly plant the rooted seedlings of sweet potato detoxification into the nursery, cover with soil, sprinkle water, adopt in time to build a plastic film shed, and add a sunshade net to cover, shade and moisturize for 20 ...

Embodiment 3

[0021] The method of transplanting sweet potato virus-free test tube seedlings directly into the soil using the stem apex meristem culture technology, the sweet potato stem section seedlings were first treated with 0.10% Hgcl 2 After 8-10 minutes of disinfection, use MS+sucrose 30g / L+agar 7.0g / L+6-BA (6-benzylaminopurine) 0.25~2.0mg / L+NAA (naphthaleneacetic acid) 0.01mg / L) medium, Induce the stem tip (0.2~0.4mm) to differentiate into seedlings, and after the strains formed are tested to be virus-free by grafting of Brazilian cows, 1 / 4 macronutrients + 1 / 2 microelements (including vitamins, iron salts) + 1 / 2 white Rapid propagation of test-tube plantlets in granulated sugar medium. Grow to 5 leaves and form the rooted seedlings of sweet potato detoxification, directly plant the rooted seedlings of sweet potato detoxification into the nursery, cover with soil, sprinkle water, adopt in time to build a plastic film shed, and add a sunshade net to cover, shade and moisturize for 20...

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Abstract

The invention relates to the technical field of plant test-tube seedling transplantation, in particular to a method for directly transplanting sweet potato virus-free test-tube seedlings into the soil in a net shed. For the rooted seedlings of sweet potato detoxified, plant the detoxified sweet potato rooted seedlings directly into the nursery, cover with soil, sprinkle water, build a plastic film arch shed in time, and add a sunshade net to cover, shade and moisturize for 30 days, remove the sunshade net and plastic film. The sweet potato detoxification test tube seedling netting shed method of the present invention directly transplants into the soil, saves the step of transplanting in the nutrient pot in the conventional method, not only simplifies the operation process, but also shortens the time for slowing down the seedlings. New leaves and new roots grow, and they grow vigorously after 30 days. The key is that the survival rate is greatly improved, and the survival rate reaches more than 96%.

Description

technical field [0001] The invention relates to the technical field of transplanting test-tube seedlings of plants, in particular to a method for directly transplanting sweet potato virus-free test-tube seedlings into a net shed. Background technique [0002] Sweet potato is an important root crop for food, feed, industrial raw materials and new energy, and it is also the bottom-line crop to ensure my country's food security. my country has planted 80 million mu per year, and its output accounts for more than 80% of the world's total output. However, sweet potato is a vegetatively propagated crop, which is infected by various viruses and pathogenic bacteria, resulting in degeneration of species, lower yield and lower quality. At present, there are more than 20 kinds of viruses and virus-like viruses that infect sweet potato, especially sweet potato virus disease (SPVD). Seedlings are the only way to remove viruses, restore the good traits of varieties, and improve the yield...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01H4/00
Inventor 李江辉武宗信解红娥解晓红王凌云陈丽李波
Owner COTTON RES INST SHANXI ACAD OF AGRI SCI
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