A method for tissue culture and propagation of Chrysanthemum chrysanthemum

A technology of tissue culture and Chrysanthemum chrysanthemum, applied in the field of plant tissue culture, can solve the problems of affecting the excellent genetic traits of seedlings, prone to variation in proliferation, and inability to effectively remove viruses, etc., to improve tissue culture procedures, increase differentiation and proliferation rate, virus less effect

Inactive Publication Date: 2016-01-27
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, the research reports on the tissue culture of Chrysanthemum chrysanthemum both at home and abroad all use leaves, petioles, hypocotyls, stems and petals as explants for tissue culture of Chrysanthemum chrysanthemum (Luo Zijuan, 1987; Zhang Yuan, 2008; Zhi Hui, 2004 ; Niu Yanbing, 2007; Zhang Na, 2009), but none of these methods can effectively remove the virus in the body of Chrysanthemum chrysanthemum. The tissues differentiate into adventitious buds, and the adventitious buds obtained through this process are prone to variation during the multiplication process, which affects the original excellent genetic traits of the seedlings

Method used

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  • A method for tissue culture and propagation of Chrysanthemum chrysanthemum
  • A method for tissue culture and propagation of Chrysanthemum chrysanthemum
  • A method for tissue culture and propagation of Chrysanthemum chrysanthemum

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Experimental program
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Effect test

Embodiment 1

[0036] (1) Field selection: Select the chrysanthemum that grows well and has no pests and diseases in the chrysanthemum base in Tongxiang, Zhejiang.

[0037] (2) Acquisition of sterile materials and induction culture: Cut out the young axillary buds of Chrysanthemum chrysanthemum with good growth and no pests and diseases, rinse with tap water for 0.5h, then shake for 2mins in an aqueous solution with a volume concentration of 1% detergent, and use Rinse with sterile water; soak 30s in 70% volume concentration ethanol water solution on the ultra-clean workbench, then soak in mixed disinfectant solution for 10mins, and then rinse with sterile water for 3 times; dry the water on sterilized filter paper, and use Peel off the 0.3mm axillary bud tip with a scalpel, then inoculate the axillary bud tip into a culture bottle filled with cluster bud induction medium, cover the bottle cap and seal the bottle mouth with a parafilm, and place it in an incubator at 24°C under light Cultiva...

Embodiment 2

[0041] The influence of embodiment 2 disinfectants on the growth of Chrysanthemum explants

[0042] 10% hydrogen peroxide (H 2 o 2 ) aqueous solution and 0.1% mercuric chloride (HgCl 2) two disinfectants in aqueous solution to replace the mixed disinfectant in Step (2) of Example 1 to disinfect the explant material (ie, axillary buds) of Chrysanthemum chrysanthemum, and other operations are the same as in Example 1. Observe and count the effects of different disinfectants on buds of Chrysanthemum The effects of induction and growth (i.e. observing the growth of axillary bud tips on cluster bud induction medium), the results are shown in Table 1. The results showed that the mixed disinfectant and mass concentration 0.1%HgCl 2 The disinfection effect is basically the same, the disinfection effect of hydrogen peroxide with a mass concentration of 10% is slightly worse, the pollution rate reaches 10%, and the growth is slow. But the mass concentration is 0.1%HgCl 2 Disinfecti...

Embodiment 3

[0045] Example 3 Different Hormone Combinations Effects on the Induction of Chrysanthemum Chrysanthemum Buds

[0046] The axillary buds of Chrysanthemum chrysanthemum were respectively inoculated in MS basic medium (ie medium for bud formation induction) supplemented with different concentrations of 6-BA and NAA. The results showed that the medium with different combinations of hormones could induce the formation of shoots to varying degrees. Among them, the medium MS+6-BA1.5mg / L+NAA0.2mg / L+100mg / L glutamine is the best, the most regenerated buds are induced, the growth is fast, the average bud length is 2.05cm, and the bud induction rate reaches 100%, the seedlings are large and strong, without vitrification, and the incision parts of the culture medium without glutamine all show different degrees of browning.

[0047] Table 2 Effects of Different Hormone Concentrations on the Induction of Hangbai Chrysanthemum Buds

[0048]

[0049]

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Abstract

The invention discloses a Chrysanthemum morifolium tissue culturing and breeding method. The method enables a large amount of high-quality Chrysanthemum morifolium seedlings to be obtained within a short period to be obtained in order to meet the needs of the market. The method comprises the steps of aseptic material obtaining, inductive culturing bud propagation and rooting, strong seedling culturing, and rooted seedling transplanting. Axillalry buds are used as an explants, and the tissue culturing method is used to rapidly breed, so the disadvantages of long period, low breeding coefficient, easy infection and propagation of viruses, and the like of routine cutting and division breeding of Chrysanthemum morifolium are overcome; and tissue culture seedlings produced by using the method have the advantages of few viruses, stable heredity and the like.

Description

(1) Technical field [0001] The invention relates to plant tissue culture technology, in particular to a method for tissue culture and rapid seedling propagation of Chrysanthemum chrysanthemum, through which large-scale production of excellent seedlings can be carried out. (2) Background technology [0002] Hangbaiju (Chrysanthemummorifolium Ramat) is a medicinal and edible herb of Compositae produced in Zhejiang, my country. It is one of the eight famous medicinal materials "Zhejiang Eight Flavors" in Zhejiang Province. Chrysanthemum chrysanthemum can be used not only as a medicine, but also as a beverage for drinking, and as a garden ornamental. Chrysanthemum contains volatile oil, flavonoids, polyphenols, inulin, purine, choline, stachydrine, vitamin A, vitamin B and amino acids. The study of traditional Chinese and Western medicine shows that Chrysanthemum chrysanthemum has the functions of dispelling wind and clearing heat, calming the liver and improving eyesight, deto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 邹克琴李素芳王为民张兴涛
Owner CHINA JILIANG UNIV
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