Method for preparing polycaprolactone-keratin composite nanometer fiber pipe

A polycaprolactone and keratin technology, applied in textiles, papermaking, non-woven fabrics, etc., can solve problems such as unfavorable endothelial cell adhesion and growth, low mechanical properties of single keratin fibers, and lack of cell recognition signal sites. It can achieve good biological activity, simple preparation method and good biocompatibility.

Inactive Publication Date: 2014-08-27
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the polycaprolactone material itself has no biological activity and lacks cell recognition signal sites, which is not conducive to the adhesion and growth of tissue cells such as endothelial cells
[0004] Accordingly, the present invention proposes to compound keratin and polycaprolactone, and prepare nanofibrous tubular materials by electrospinning, in order to improve the low mechanical properties of single keratin fiber materials.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The keratin was extracted from wool by reduction method. The specific method was: weigh 5g of wool, use petroleum ether as solvent to remove the oil on the surface of wool by Soxhlet extraction, then wash the wool with ethanol and air-dry it. Add the above-mentioned wool into 100ml, 1mol / l mercaptoethanol, adjust the pH to about 10.0 with NaOH, and then react at 40°C for 12h. After the reaction was finished, the reaction solution was collected (referred to as filtrate 1), and the wool solid remaining in the reaction was collected by filtration, and continued to be treated with 100ml, 0.1mol / l Tris lye at 40°C for 2h, and then filtered to remove the solid. The filtrate was collected (designated as filtrate 2). .The filtrates 1 and 2 were combined, centrifuged at 6000rpm for 5 minutes, and then dialyzed with a dialysis bag with a molecular weight cut-off of 40,000 for 36 hours. Finally, the dialysate was added to liquid nitrogen for quick freezing, and then dried in a fre...

Embodiment 2

[0028] Using human hair as raw material to extract keratin by reduction method, the specific method is: weigh 5g of human hair, use petroleum ether as solvent to remove human hair surface oil by Soxhlet extraction, then wash human hair with ethanol and air-dry. Add the above-mentioned human hair into a mixed solution of 200ml of water and 5g of sodium metabisulfite, adjust the pH to about 10.0 with NaOH, and then react at 60°C for 5h. After the reaction, collect the reaction solution (referred to as filtrate 1), and collect the remaining human hair solid by filtration at the same time, continue to treat it with 100ml, 0.1mol / l Tris lye at 40°C for 2h, and then filter to remove the solid , collect the filtrate (referred to as filtrate 2). .The filtrates 1 and 2 were combined, centrifuged at 6000rpm for 5 minutes, and then dialyzed with a dialysis bag with a molecular weight cut-off of 8,000 for 36 hours. Finally, the dialysate was added to liquid nitrogen for quick freezing, an...

Embodiment 3

[0031]The keratin is extracted from wool by oxidation method. The specific method is: weigh 5g of wool, use petroleum ether as solvent to remove the oil on the surface of wool by Soxhlet extraction, then wash the wool with ethanol and air-dry it. 100ml of deionized water and 5ml of 30% hydrogen peroxide were added to the wool, and then reacted at 100°C for 2h. After the reaction was finished, the reaction solution was collected (referred to as filtrate 1), and the wool solid remaining in the reaction was collected by filtration, and continued to be treated with 100ml, 0.1mol / l Tris lye at 40°C for 2h, and then filtered to remove the solid. The filtrate was collected (designated as filtrate 2). .The filtrates 1 and 2 were combined, centrifuged at 6000rpm for 5 minutes, and then dialyzed with a dialysis bag with a molecular weight cut-off of 8,000 for 36 hours. Finally, the dialysate was added to liquid nitrogen for quick freezing, and then dried in a freeze dryer to obtain dry ...

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PUM

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Abstract

The invention relates to a method for preparing a polycaprolactone-keratin composite nanometer fiber pipe. The method includes the steps of firstly, dissolving polycaprolactone in a solvent, stirring the solvent to dissolve the polycaprolactone, and obtaining a polycaprolactone solution; secondly, dissolving keratin in a solvent, stirring the solvent to dissolve the keratin, and obtaining a keratin solution; thirdly, mixing the polycaprolactone solution with the keratin solution according to the mass ratio of 95:5 to 5:95, stirring the mixture to mix the mixture evenly, and obtaining a spinning solution; fourthly, conducting electrostatic spinning, and obtaining the polycaprolactone-keratin composite nanometer fiber pipe with a cylindrical collection roller serving as a receiver. The tubular material obtained through the method not only keeps good biological activity of the keratin, but also has good tenacity and mechanical strength of the polycaprolactone; meanwhile, the tubular material has the advantages of being adjustable in inner diameter, simple in preparation method and the like and is expected to be used for the field of artificial blood vessels and the like.

Description

technical field [0001] The invention belongs to the field of preparation of nanofiber tubes, in particular to a preparation method of polycaprolactone-keratin composite nanofiber tubes. Background technique [0002] Keratin is an insoluble fibrous animal protein, a structural protein of ectodermal cells, widely present in animal skin and skin appendages, such as hair, hooves, shells, claws, horns, scales, etc. A large number of studies in recent years have shown that keratin is a high-quality biomedical material with good biocompatibility and not rejected by the body's immune system, and has broad application prospects. Most prominently, the amino acid sequence of keratin derived from wool and other sources was found to contain the same Arg-Gly-Asp (RGD) tripeptide sequence. This tripeptide sequence is recognized as an effective binding site for cell binding in the extracellular matrix, and has the function of promoting cell adsorption. Therefore, a large number of basic r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D04H1/4382D04H1/728D04H1/76
Inventor 杨光曹张军杨雪霞
Owner DONGHUA UNIV
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